Identification of putative biomarkers for the serodiagnosis of drug-resistant Mycobacterium tuberculosis.

Proteome Science
Lu ZhangHonghai Wang

Abstract

Early diagnosis and treatment of Mycobacterium tuberculosis infection can prevent most deaths resulting from this pathogen; however, multidrug-resistant strains present serious threats to global tuberculosis control and prevention efforts. In this study, we identified antigens that could be used for the serodiagnosis of drug-resistant M. tuberculosis strains, using a proteomics-based analysis. Serum from patients infected with drug-resistant or drug-susceptible M. tuberculosis strains and healthy controls was subjected to two-dimensional gel electrophoresis using a western blot approach. This procedure identified nine immunoreactive proteins, which were subjected to MALDI-TOF-MS analysis. Six recombinant proteins, namely rRv2031c, rRv0444c, rRv2145c, rRv3692, rRv0859c, and rRv3040, were expressed and used to determine the immuno-reactivity of 100 serum samples. Antibody reactivity against rRv2031c, rRv3692, and rRv0444c was consistently observed. Among them, the best sensitivity and specificity of rRv3692 were 37% and 95% respectively. Furthermore, when rRv2031c and rRv3692 or rRv2031c, rRv3692, and rRv0444c were combined in 2:1 or equal amounts, the assay sensitivity and specificity were improved to 56.7% and 100% respectively...Continue Reading

References

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Citations

Feb 18, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Colleen A BurgeRebecca Vega-Thurber
Aug 13, 2013·Clinics in Laboratory Medicine·Robert F Luo, Niaz Banaei
Sep 17, 2013·Journal of Proteomics·Bhavnesh KumarDeepa Bisht
Jan 24, 2014·Pathogens and Disease·Patrick W KernsMark E Shirtliff
Nov 1, 2016·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·Natasha Naidoo, Manormoney Pillay
Feb 21, 2018·Interdisciplinary Sciences, Computational Life Sciences·Caroline KönigJesús Giraldo
Apr 30, 2019·Expert Review of Proteomics·Deepa BishtVivek Kumar Gupta

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Methods Mentioned

BETA
ELISA
electrophoresis
X-ray
PCR

Software Mentioned

MASCOT
PDQuest
ProtParam
GPS Explorer
Statistics Package for Social Science ( SPSS

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