Identification of putative biomarkers for toluene-degrading Burkholderia and pseudomonas by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and peptide mass fingerprinting

Bioscience, Biotechnology, and Biochemistry
Erica M HartmannRolf U Halden

Abstract

The use of peptide mass fingerprinting with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) was demonstrated to identify and phenotypically characterize toluene-degrading bacteria via biomarkers of degradation and taxonomical classification. Pseudomonas putida F1, P. mendocina KR1, and Burkholderia sp. JS150 were grown on toluene, extracted, electrophoretically separated, and analyzed by MALDI-TOF MS. Catabolic enzymes were identified and results substantiated using tandem MS.

References

Feb 9, 2002·Mass Spectrometry Reviews·C Fenselau, P A Demirev
Dec 13, 2006·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Catherine Fenselau

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioremediation (ASM)

Bioremediation is the treatment and removal of harmful pollutants or contaminants through the use of microorganisms. Discover the latest research here.