Identification of putative drug targets in Vancomycin-resistant Staphylococcus aureus (VRSA) using computer aided protein data analysis

Gene
Md Anayet HasanAfrin Sultana Chowdhury

Abstract

Vancomycin-resistant Staphylococcus aureus (VRSA) is a Gram-positive, facultative aerobic bacterium which is evolved from the extensive exposure of Vancomycin to Methicillin resistant S. aureus (MRSA) that had become the most common cause of hospital and community-acquired infections. Due to the emergence of different antibiotic resistance strains, there is an exigency to develop novel drug targets to address the provocation of multidrug-resistant bacteria. In this study, in-silico genome subtraction methodology was used to design potential and pathogen specific drug targets against VRSA. Our study divulged 1987 proteins from the proteome of 34,549 proteins, which have no homologues in human genome after sequential analysis through CD-HIT and BLASTp. The high stringency analysis of the remaining proteins against database of essential genes (DEG) resulted in 169 proteins which are essential for S. aureus. Metabolic pathway analysis of human host and pathogen by KAAS at the KEGG server sorted out 19 proteins involved in unique metabolic pathways. 26 human non-homologous membrane-bound essential proteins including 4 which were also involved in unique metabolic pathway were deduced through PSORTb, CELLO v.2.5, ngLOC. Functional cla...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Aug 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M A HedigerE M Wright
Apr 1, 1988·The Journal of Antimicrobial Chemotherapy·G Gialdroni Grassi
Sep 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·I T ChenD J Roufa
Jan 11, 1985·Nucleic Acids Research·J S Miles, J R Guest
Feb 21, 1967·Biochemical and Biophysical Research Communications·K H SchleiferO Kandler
Nov 1, 1993·Antimicrobial Agents and Chemotherapy·N C ClarkF C Tenover
Jul 1, 1997·The Journal of Antimicrobial Chemotherapy·K HiramatsuF C Tenover
Aug 26, 1998·The New England Journal of Medicine·F D Lowy
Jan 9, 1999·The New England Journal of Medicine·S BlotF Colardyn
Mar 19, 1999·The Journal of Infectious Diseases·H F Chambers
Nov 5, 1999·Trends in Microbiology·M O Clements, S J Foster
Dec 11, 1999·Nucleic Acids Research·M Kanehisa, S Goto
Mar 4, 2000·Proceedings of the National Academy of Sciences of the United States of America·F R StermitzK Lewis
Mar 27, 2001·FEMS Microbiology Letters·U StrychM J Benedik
Jun 13, 2001·Molecular Microbiology·S K MazmanianO Schneewind
Sep 10, 2002·Trends in Biochemical Sciences·Corin YeatsAlex Bateman
Sep 14, 2002·Current Infectious Disease Reports·Ambrose L. CheungHattie Gresham
Oct 1, 1964·Proceedings of the National Academy of Sciences of the United States of America·W KUNDIGS ROSEMAN
Dec 19, 2003·Nucleic Acids Research·Ren ZhangChun-Ting Zhang
Jul 1, 1951·Biochimica Et Biophysica Acta·M R J SALTON, R W HORNE
Jun 25, 2004·Nucleic Acids Research·Pantelis G BagosStavros J Hamodrakas
Aug 13, 2004·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Hilmar WisplinghoffMichael B Edmond
Oct 23, 2004·The Journal of Infectious Diseases·Heather A CarletonFrançoise Perdreau-Remington
Oct 27, 2004·Antimicrobial Agents and Chemotherapy·Bruno Périchon, Patrice Courvalin
Jan 7, 2005·Journal of Clinical Microbiology·I C V PalazzoA L C Darini
Oct 11, 2005·Computational Biology and Chemistry·Sharmila AnishettyGautam Pennathur
Dec 31, 2005·Nucleic Acids Research·David S WishartJennifer Woolsey
Mar 2, 2006·Biological & Pharmaceutical Bulletin·Youichi YamadaTomofusa Tsuchiya

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Citations

Dec 7, 2016·Frontiers in Microbiology·Mohammad Uzzal HossainMd Salimullah
May 28, 2020·International Journal of Environmental Research and Public Health·Farah ShahidMohsin Khurshid

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