Identification of putative negative regulators of yeast signaling through a screening for protein phosphatases acting on cell wall integrity and mating MAPK pathways

Fungal Genetics and Biology : FG & B
Almudena Sacristán-ReviriegoMaría Molina

Abstract

The lack of signaling through MAPK pathways leads to a defective cellular response to the corresponding stimulus, but an improper hyperactivation of these routes results in deleterious effects as well. Protein phosphorylation is an activating modification for signal transmission through components of MAPK pathways and thus, protein phosphatases are key negative regulators of these cellular routes by limiting excessive signaling activity. However, in contrast to most of the protein kinases operating in MAPK pathways, protein phosphatases usually exhibit redundancy and promiscuity, which has limited the identification of their function. In order to identify new putative phosphatases operating in Saccharomyces cerevisiae MAPK signaling, we have taken advantage of growth inhibition promoted by overproduction of constitutively active components of the mating and cell wall integrity (CWI) pathways to perform a screen with a collection of 43 protein phosphatases or phosphatase-regulatory proteins. The phosphatases able to alleviate the induced growth inhibition when overproduced were further studied by testing their capacity to downregulate expression of mating and CWI responsive promoters and the consequences of their removal on MAPK...Continue Reading

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Oct 7, 2015·Fungal Genetics and Biology : FG & B·Ulrich KückInes Teichert
Jul 13, 2016·FEMS Yeast Research·Jacqueline PérezCésar Roncero
May 21, 2020·Molecular & Cellular Proteomics : MCP·Cynthia CheliusMark R Marten
Feb 6, 2019·Frontiers in Physiology·Niek WelkenhuysenMarija Cvijovic
Oct 11, 2020·Research in Microbiology·Ján Víglaš, Petra Olejníková
Jan 17, 2021·Molecular & Cellular Proteomics : MCP·Cynthia CheliusMark R Marten

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