Identification of putative parasitism genes expressed in the esophageal gland cells of the soybean cyst nematode Heterodera glycines

Molecular Plant-microbe Interactions : MPMI
B GaoR S Hussey

Abstract

Cloning parasitism genes encoding secretory proteins expressed in the esophageal gland cells is the key to understanding the molecular basis of nematode parasitism of plants. Suppression subtractive hybridization (SSH) with the microaspirated contents from Heterodera glycines esophageal gland cells and intestinal region was used to isolate genes expressed preferentially in the gland cells of parasitic stages. Twenty-three unique cDNA sequences from a SSH cDNA library were identified and hybridized to the genomic DNA of H. glycines in Southern blots. Full-length cDNAs of 21 clones were obtained by screening a gland-cell long-distance polymerase chain reaction cDNA library. Deduced proteins of 10 clones were preceded by a signal peptide for secretion, and PSORT II computer analysis predicted eight proteins as extracellular, one as nuclear, and one as plasmalemma localized. In situ hybridization showed that four of the predicted extracellular clones were expressed specifically in the dorsal gland cell, one in the subventral gland cells, and three in the intestine in H. glycines. The predicted nuclear clone and the plasmalemma-localized clone were expressed in the subventral gland cells and the dorsal gland cell, respectively. SSH ...Continue Reading

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Citations

May 20, 2005·Molecular Genetics and Genomics : MGG·N AtibalentjaK N Lambert
Mar 24, 2004·Trends in Parasitology·Eric L DavisThomas J Baum
Sep 3, 2002·International Journal for Parasitology·Bingli GaoRichard S Hussey
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Jan 1, 2003·Molecular Plant Pathology·John T JonesAlison Prior
Nov 6, 2009·Journal of Experimental Botany·Nrupali PatelEric L Davis
Mar 24, 2007·Molecular Plant-microbe Interactions : MPMI·Nagabhushana IthalMelissa G Mitchum
Jun 13, 2002·Molecular Plant-microbe Interactions : MPMI·Elizabeth A Doyle, Kris N Lambert
Dec 4, 2003·Molecular Plant-microbe Interactions : MPMI·Cédric NeveuPhilippe Castagnone-Sereno
Feb 11, 2003·Molecular Plant-microbe Interactions : MPMI·Elizabeth A Doyle, Kris N Lambert
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Guozhong HuangRichard S Hussey
May 15, 2003·Molecular Plant-microbe Interactions : MPMI·Sadia BekalKris N Lambert
Aug 9, 2003·Molecular Plant-microbe Interactions : MPMI·Bingli GaoRichard S Hussey
Jul 31, 2002·Annual Review of Phytopathology·Godelieve Gheysen, Carmen Fenoll
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