Identification of putative transcription factor binding sites in rodent selenoprotein W promoter

Journal of Inorganic Biochemistry
A AmantanaP D Whanger

Abstract

To understand transcriptional regulation of the selenoprotein W (SeW) gene, we used in vitro binding assays to identify transcription factors that may be involved in the transcriptional regulation of the SeW gene. Using protein from rat C6 (glial) cell nuclear extracts, oligonucleotides containing putative regulatory elements in the SeW promoter and antibodies, we observed that specificity protein 1(Sp1) transcription factor binds to the Sp1 consensus sequence in the SeW promoter as well as to the metal response element (MRE). Although competition analysis showed specific binding at the TFII-1 site, super-shift analysis using anti-TFII-1 antibody did not yield any super-shifted band. Therefore, the SeW gene may be a target for Sp1 whose binding to various regulatory sequences of the SeW promoter may activate or repress the transcription of SeW. The MRE, GRE, AP-1 and LF-A1 sites were also tested but no evidence was obtained for specific binding as indicated by lack of competition with unlabeled probes.

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Citations

Oct 14, 2005·Nucleic Acids Research·Ursula WimmerWalter Schaffner
Oct 20, 2010·The Journal of Biological Chemistry·Ok Jeong NohIck Young Kim
May 19, 2007·Antioxidants & Redox Signaling·Laura Vanda PappKum Kum Khanna
May 26, 2009·Biochimica Et Biophysica Acta·P D Whanger
May 26, 2009·Biochimica Et Biophysica Acta·Zoia R Stoytcheva, Marla J Berry
Nov 9, 2005·Prostate Cancer and Prostatic Diseases·S R J BottM Williamson
Nov 5, 2013·Metallomics : Integrated Biometal Science·Marco RomanCarlo Barbante

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