Identification of RB18A, a 205 kDa new p53 regulatory protein which shares antigenic and functional properties with p53

Oncogene
P DranéR Frade

Abstract

Immunological screening with the anti-p53 moAb, PAb1801 of a cDNA expression library, prepared from human B lymphoma cells, led us to identify a new human 205 kDa protein called RB18A for 'Recognized By PAb1801 moAntibody'. Immunoblotting or immunoprecipitation of fusion protein or in vitro translated protein, respectively, demonstrated that RB18A protein was recognized by several anti-p53 moAb reacting with the N or C-terminal domains of p53. Full length sequence of RB18A cDNA and computer analysis demonstrated that despite common antigenic determinants between RB18A and p53 proteins, nucleotide and deduced protein sequences did not reveal any significant homologies. RB18A mRNA was detected in all tissues tested except in kidney. In addition, RB18A protein shared identical functions with p53 protein: binding to DNA or to p53 and self-oligomerization. Furthermore, RB18A regulated p53 specific binding on his DNA consensus binding site. These functions were associated to the C-terminal domain of RB18A protein and more specifically to the PAb421 binding site present in this domain. The activation by RB18A of p53 binding on DNA was induced through an unstable interaction between both proteins. Altogether, our data demonstrated that...Continue Reading

Citations

Feb 4, 2005·Chromosoma·Erik BlazekMichael Meisterernst
Aug 4, 2005·The Surgeon : Journal of the Royal Colleges of Surgeons of Edinburgh and Ireland·R J C Steele, D P Lane
May 11, 2010·Nature Structural & Molecular Biology·Krista D MeyerDylan J Taatjes
Nov 21, 1998·The British Journal of Surgery·R J SteeleD P Lane
Jul 22, 1998·Nucleic Acids Research·J MomandJ Niland
Jul 13, 2000·Molecular and Cellular Biology·Y RenJ D Fondell
Jun 8, 2001·Annual Review of Biochemistry·A M NäärR Tjian
Sep 4, 2010·PPAR Research·Navin ViswakarmaJanardan K Reddy
Sep 15, 1999·Proceedings of the National Academy of Sciences of the United States of America·Y ZhuJ K Reddy
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·S Ryu, R Tjian
Apr 26, 2005·FEBS Letters·Séverine Lottin-DivouxRaymond Frade
Feb 27, 2009·International Journal of Cancer. Journal International Du Cancer·Jean de La Croix NdongRaymond Frade
Oct 4, 2013·Critical Reviews in Biochemistry and Molecular Biology·Zachary C PossDylan J Taatjes
Aug 26, 2011·Seminars in Cell & Developmental Biology·David Ries, Michael Meisterernst
Nov 12, 2016·Cold Spring Harbor Perspectives in Medicine·Neil T Pfister, Carol Prives
Nov 11, 2017·Cell Death and Differentiation·Kelly D SullivanJoaquin M Espinosa
Jun 24, 2017·Arteriosclerosis, Thrombosis, and Vascular Biology·Liang BaiEnqi Liu
May 26, 1999·The Journal of Pathology·C Prives, P A Hall
Sep 9, 1999·Molecular Endocrinology·G B AtkinsM A Lazar
Feb 13, 2002·Oncogene·Raymond FradeMonique Barel
Oct 27, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Min-Sung LeeSeung-Wook Chi
Jan 1, 2018·Cell Death and Differentiation·Kelly D SullivanJoaquin M Espinosa
Aug 16, 2011·Seminars in Cell & Developmental Biology·Tilman Borggrefe, Xiaojing Yue

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.