Identification of regions in HIV-1 Nef required for efficient downregulation of cell surface CD4

Virology
J HuaB R Cullen

Abstract

Downregulation of cell surface CD4 is a characteristic property of all lentiviral Nef proteins. We have used mutational analysis to define regions within HIV-1 Nef that are critical for this biological activity. Two discontinuous regions in Nef, extending approximately from residues 96 to 144 and from residues 175 to 186, are reported to be essential for efficient CD4 downregulation. Interestingly, these sequences coincide with two conserved regions of the Nef protein that are juxtaposed to form a single surface on the known structure of Nef. A third, more amino terminal conserved region in Nef, previously reported to be important for Nef enhancement of virion infectivity, was found to be largely dispensable for CD4 downregulation. These data raise the possibility that Nef may contain two structurally distinct functional domains, only one of which contributes to the CD4 downregulation phenotype.

References

Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·E Zazopoulos, W A Haseltine
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·S R HammesW C Greene
Aug 1, 1995·Journal of Virology·C Aiken, D Trono
Aug 1, 1993·Journal of Virology·D C ShugarsR Swanstrom
Jun 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R Mariani, J Skowronski

❮ Previous
Next ❯

Citations

Dec 3, 1999·European Journal of Clinical Investigation·F SilvestrisF Dammacco
Jan 21, 2010·Antimicrobial Agents and Chemotherapy·Roger G PtakJohn C Drach
May 1, 2009·Journal of Virology·Luis L P daSilvaJuan S Bonifacino
Feb 2, 2013·PloS One·Mohamed El-FarRafick-Pierre Sékaly
Sep 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·H M CraigJ C Guatelli
Jan 30, 2014·ELife·Xuefeng RenJames H Hurley
May 18, 1999·Archives of Biochemistry and Biophysics·J W Marsh
Sep 12, 2015·Journal of Virology·Aniqa ShahidZabrina L Brumme
Jul 29, 2020·Nature Structural & Molecular Biology·Yonghwa KwonXiaofei Jia
Jul 28, 2020·BMC Infectious Diseases·Samara Tatielle Monteiro GomesAntonio Carlos Rosário Vallinoto
Apr 2, 2002·Biochemical and Biophysical Research Communications·Andrea PreusserDieter Willbold

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.