Identification of reliable reference genes for quantitative real-time PCR in ovary and uterus of laying hens under heat stress

Stress : the International Journal on the Biology of Stress
Hossein HassanpourW Kaewduangta

Abstract

The main stage in real-time quantitative PCR is a quantification of gene transcriptomes, in which suitable use of reliable reference genes is critical to normalize accurately. To determine the most stable reference genes in laying hens under heat stress, from a panel of nine typical candidate reference genes, the mRNA transcript of ACTB, HMBS, HPRT1, RPL13, RPL32, 18SrRNA, TBP, TFRC, and YWHAZ was evaluated in the ovary and uterus of both control and heat-stress groups of laying hens. Forty 23-week-old White Leghorn laying hens were housed in two rooms. The control (n = 20) and heat-stress (n = 20) groups were maintained at 21-23 °C and 36-38 °C for 8 weeks respectively. Analysis of this set of genes was done with BestKeeper, geNorm, and NormFinder software programs to find the most stable ones. Candidate reference genes ranked in the uterus of heat-stress and control groups of hens included YWHAZ, HPRT1, HMBS, RPL13, TFRC, ACTB, TBP, RPL32, and 18SrRNA; those in the ovary were YWHAZ, HPRT1, TFRC, HMBS, RPL13, TBP, RPL32, ACTB, and 18SrRNA. The overall results indicated that the most stable genes are YWHAZ, HPRT1, HMBS, RPL13, TFRC, TBP, ACTB, RPL32, and 18SrRNA respectively. In addition, the combination of YWHAZ, HPRT1, and HM...Continue Reading

References

Aug 19, 2000·BioTechniques·T SuzukiD R Crawford
Mar 16, 2002·Journal of Applied Physiology·Larry A SonnaCraig M Lilly
Jul 31, 2004·BioTechniques·Keertan DhedaAlimuddin Zumla
Aug 3, 2005·BioTechniques·Marisa L Wong, Juan F Medrano
Feb 8, 2006·Molecular Aspects of Medicine·Mikael KubistaNeven Zoric
Jul 14, 2007·Poultry Science·I RozenboimZ Uni
Feb 12, 2009·The Biochemical Journal·Raj GillPeter M Shoolingin-Jordan
Nov 13, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Krishna R HamalGisela F Erf
Jun 16, 2011·Poultry Science·Z W ZhangX L Wang
Jul 14, 2011·Journal of Animal Breeding and Genetics = Zeitschrift Für Tierzüchtung Und Züchtungsbiologie·Y R GuX W Li
May 24, 2012·Journal of Biomedicine & Biotechnology·Zhigang SongHai Lin
Sep 10, 2013·International Journal of Nursing Education Scholarship·Deborah A Raines
Oct 1, 2013·Journal of Applied Genetics·Bartłomiej Kozera, Marcin Rapacz
Jun 24, 2014·Archives of Biochemistry and Biophysics·Myrka Jérôme, Hemant K Paudel
Mar 17, 2015·Journal of Animal Science and Biotechnology·Hongyan SunLujiang Qu
Jan 1, 2013·Animals : an Open Access Journal From MDPI·Lucas J Lara, Marcos H Rostagno
Feb 14, 2016·Veterinary Immunology and Immunopathology·D BorowskaP Kaiser
Sep 26, 2017·Environmental Toxicology and Pharmacology·Dorota Katarzyńska-BanasikAndrzej Sechman
Nov 9, 2017·Stress : the International Journal on the Biology of Stress·Shahab BahadoranHossein Hassanpour

❮ Previous
Next ❯

Citations

Aug 26, 2020·PloS One·Ludmila Mudri HulMônica Corrêa Ledur
Jan 18, 2021·Molecular Biology Reports·Fabiana Cristina Belchior de SousaKatiene Régia Silva Sousa
May 21, 2021·Veterinary Medicine and Science·Roy Rodríguez-HernándezIang S Rondón-Barragán

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.