Identification of repressive and active epigenetic marks and nuclear bodies in Entamoeba histolytica

Parasites & Vectors
Daniela Lozano-AmadoRosaura Hernández-Rivas

Abstract

In human hosts, Entamoeba histolytica cysts can develop into trophozoites, suggesting that the life cycle of this parasite are regulated by changes in gene expression. To date, some evidence has suggested that epigenetic mechanisms such as DNA methylation and histone modification are involved in the regulation of gene expression in Entamoeba. Some post-translational modifications (PTMs) at the N-terminus of E. histolytica's histones have been reported experimentally, including tri-methylation in the lysine 4 of histone H3 (H3K4me3) and dimethylation in the lysine 27 of histone H3 (H3K27me2), dimethylation of arginine 3 (H4R3me2) and the indirect acetylation of histone H4 in the N-terminal region. However, it is not known which residues of histone H4 are subject to acetylation and/or methylation or where in the nucleus these epigenetic marks are located. Histones from trophozoites of E. histolytica were obtained and analyzed by LC-MS/MS. WB assays were performed using antibodies against epigenetic marks (acetylated lysines and methylated arginines). Immunofluorescence assays (IFA) were carried out to determine the distribution of PTMs and the localization of DNA methylation as a heterochromatin marker. Nuclear bodies such as the...Continue Reading

References

Jan 1, 1978·Transactions of the Royal Society of Tropical Medicine and Hygiene·L S DiamondC C Cunnick
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1991·Molecular and Biochemical Parasitology·H Torres-GuerreroI Meza
May 1, 1995·Molecular and Biochemical Parasitology·M BinderM Duchêne
Aug 3, 2002·The Journal of Biological Chemistry·Bettina SargHerbert H Lindner
Nov 26, 2002·Trends in Cell Biology·Maria Carmo-FonsecaJason R Swedlow
Nov 25, 2003·Analytical Chemistry·Alexey I NesvizhskiiRuedi Aebersold
May 7, 2004·Journal of Cell Science·Niki KourmouliPrim B Singh
Nov 24, 2004·Molecular and Biochemical Parasitology·Girija RamakrishnanWilliam A Petri
Jan 22, 2005·Cellular Microbiology·Jennifer ByersDaniel Eichinger
Feb 25, 2005·Nature·Brendan LoftusNeil Hall
Nov 24, 2005·Journal of Cell Science·Joanna E LowellGeorge A M Cross
Oct 10, 2006·Cellular Microbiology·William J SullivanSergio O Angel
May 8, 2007·Trends in Microbiology·Miguel NavarroDavid Landeira
Nov 21, 2007·Cell·Tony Kouzarides
May 22, 2008·IUBMB Life·David MirelmanRivka Bracha
May 7, 2009·Molecular and Biochemical Parasitology·Gagan Deep JhinganSudha Bhattacharya
Mar 6, 2010·Journal of Biomedicine & Biotechnology·Consuelo GomezMario A Rodríguez
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Liliana Mancio-SilvaArtur Scherf
Oct 12, 2010·Cold Spring Harbor Perspectives in Biology·David L Spector, Angus I Lamond
May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Maria Carolina DalmassoSergio Oscar Angel
Jun 18, 2011·Trends in Genetics : TIG·Yuntao S MaoDavid L Spector
Jan 25, 2013·Nucleic Acids Research·Stine JørgensenClaus Storgaard Sørensen
Aug 12, 2014·Journal of Proteomics·Jesús ValdésJosé Manuel Galindo-Rosales
Sep 17, 2014·Gene·Nathalie Durut, Julio Sáez-Vásquez
Dec 9, 2014·Epigenetics : Official Journal of the DNA Methylation Society·Karin Meier, Alexander Brehm

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Citations

Oct 20, 2018·Frontiers in Cellular and Infection Microbiology·Francisco Javier Rendón-GandarillaElisa Azuara-Liceaga
Dec 17, 2020·Microorganisms·Richard GuillonneauMaëlle Molmeret

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Methods Mentioned

BETA
acetylation
electrophoresis

Software Mentioned

Proteome Discoverer
Compute Pi / Mw
Proteome
! Tandem
Scaffold
Tandem

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Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.