Aug 1, 1989

Identification of residues necessary for clonally specific recognition of a cytotoxic T cell determinant

The EMBO Journal
J B RothbardW R Taylor

Abstract

The residues in an influenza nucleoprotein (NP) cytotoxic T cell determinant necessary for cytotoxic T cell (CTL) recognition, were identified by assaying the ability of hybrid peptides to sensitize a target cell to lysis. The hybrid peptides were formed by substituting amino acids from one determinant (influenza NP 147-158) for the corresponding residues of a second peptide (HLA CW3 171-182) capable of binding to a common class I protein (H-2Kd). Six amino acids resulted in partial recognition; however, the presence of a seventh improved the potency of the peptide. Five of the six amino acids were shown to be required for recognition. The spacing of the six amino acids was consistent with the peptide adopting a helical conformation when bound. The importance of each amino acid in CTL recognition and binding to the restriction element was investigated further by assaying the ability of peptides containing point substitutions either to sensitize target cells or to compete with the natural NP sequence for recognition by CTL. The T cell response was much more sensitive to substitution than the ability of the peptide to bind the restriction element. Collectively the separate strategies identified an approximate conformation and ori...Continue Reading

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Mentioned in this Paper

Pathologic Cytolysis
T-Lymphocyte
Influenza Virus Core Proteins
TC2 Cells
Synapsin I
Mice, Inbred BALB C
Nucleoproteins
Amino Acids, I.V. solution additive
Influenza virus vaccine
Antigenic Specificity

About this Paper

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