Identification of Riemerella anatipestifer genes differentially expressed in infected duck livers by the selective capture of transcribed sequences technique

Avian Pathology : Journal of the W.V.P.A
Zutao ZhouZili Li

Abstract

Riemerella anatipestifer is the causative agent of duck septicaemia. Determination of R. anatipestifer virulence mechanisms will help us to effectively control this contagious agent. The differentially expressed gene profile of R. anatipestifer in infected duck livers was therefore identified and compared with in vitro cultures by selective capture of transcribed sequences analysis. A total of 48 genes were identified, of which 43 were genes that encode enzymes for amino acid biosynthesis and metabolism, intermediary metabolism, and energy metabolism, or proteins for regulatory adaptive responses, general microbial stress response, transport proteins and secreted proteinases. Five were unknown, novel genes. Eight genes representing the categories were randomly chosen and verified by real-time reverse transcriptase-polymerase chain reaction analysis. All were upregulated by R. anatipestifer in infected duck livers, with changes ranging from 1.44-fold to 4.62-fold compared with in vitro cultures. The results from the present study revealed a gene expression profile of R. anatipestifer in infected duck livers. The unknown but novel genes may be potential novel virulence factors for R. anatipestifer. In conclusion, the data from th...Continue Reading

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Datasets Mentioned

BETA
FJ031240
FJ031241
CP000139
FI166206
CP000383
FI166209
CP000685
FI166210
CP000319
CP00685

Methods Mentioned

BETA
electrophoresis
PCR
PCRs
dot-blot
environmental stress

Software Mentioned

SPSS15
BLASTn

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