Identification of rutin deglycosylated metabolites produced by human intestinal bacteria using UPLC-Q-TOF/MS

Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
Jing YangJin-ao Duan

Abstract

In this paper, rutin was metabolized by human intestinal bacteria and five isolated strains including Bacillus sp. 52, Bacteroides sp. 45, 42, 22 and Veillonella sp. 32, the metabolites were identified using ultra performance liquid chromatography/quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF/MS). As a result, Bacillus sp. 52 and Bacteroides sp. 45 could metabolize rutin to quercetin 3-O-glucoside and leucocyanidin. Bacteroides sp. 42 and Veillonella sp. 32 could convert rutin to leucocyanidin. Bacteroides sp. 22 could hydrolyze rutin to quercetin-3-O-glucoside. In order to further explain the metabolism pathway of rutin, the β-D-glucosidase and α-L-rhamnosidase activities of five strains were determined. Bacteroides sp. 22 could produce α-L-rhamnosidase but did not produce β-D-glucosidase or β-D-glucosidase activity was too low to be detected. The other four strains all demonstrated α-L-rhamnosidase and β-D-glucosidase activities. Furthermore, α-L-rhamnosidase and β-D-glucosidase activities of Veillonella sp. 32 and Bacteroides sp. 42 were higher than those of Bacteroides sp. 45 and Bacillus sp. 52. Based on these results, we can propose the deglycosylated rout of rutin: rutin was metabolized to be quercetin-3-O-gluc...Continue Reading

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Citations

Apr 26, 2013·Journal of Asian Natural Products Research·Ru-Feng WangXiu-Wei Yang
Jun 12, 2013·European Journal of Drug Metabolism and Pharmacokinetics·Chunyan ZhaoGuangji Wang
Sep 17, 2013·Expert Opinion on Drug Metabolism & Toxicology·Mi Jeong KangHye Gwang Jeong
Sep 29, 2012·Biomedical Chromatography : BMC·Linling LuJin-ao Duan
Mar 29, 2014·Molecular Nutrition & Food Research·Chia-Yu HsuChing-Feng Weng
Feb 25, 2015·Biotechnology Advances·Marcel KarabinPavel Dostalek
Jun 24, 2014·Journal of Ethnopharmacology·HanLiang GuanJinao Duan
Apr 19, 2017·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·Lin YuanLantong Zhang
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Sep 16, 2017·Drug Metabolism Reviews·Mei ZengJingqing Zhang
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Aug 11, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Huai-You WangKarl W K Tsim
Mar 18, 2021·Journal of Pharmaceutical and Biomedical Analysis·Gokhan ZenginMałgorzata Polz-Dacewicz
May 27, 2021·Journal of Agricultural and Food Chemistry·Hanieh NargehHamidreza Pazoki-Toroudi

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