PMID: 9163906May 1, 1997Paper

Identification of Saccharomyces cerevisiae GLY1 as a threonine aldolase: a key enzyme in glycine biosynthesis

FEMS Microbiology Letters
N MonschauA L Bognar

Abstract

Determination of enzyme-specific activities revealed that GLY1 encodes a threonine aldolase (TA) in Saccharomyces cerevisiae. A knock-out mutant auxotrophic for glycine lacked detectable activity. After transformation with YEp24GLY1 glycine prototrophy was restored and TA-specific activity was 16-fold higher than in the wild type. Growth experiments using glucose as the sole carbon source showed that GLY1 is more important for glycine biosynthesis than SHM1 and SHM2 encoding alternative serine hydroxymethyltransferases. On ethanol as carbon source simultaneous disruption of GLY1, SHM1 and SHM2 did not lead to glycine auxotrophy because glycine biosynthesis proceeds via alanine glyoxylate aminotransferase.

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Citations

Apr 25, 2000·Archives of Biochemistry and Biophysics·L DeSouzaA L Bognar
Aug 16, 2003·European Journal of Biochemistry·Michel FlipphiBéatrice Felenbok
Jul 4, 2007·Proceedings of the National Academy of Sciences of the United States of America·John L Hartman
Jul 4, 2008·Molecular & Cellular Proteomics : MCP·Diana R ArnettP Anthony Weil
Jul 4, 2003·Applied and Environmental Microbiology·Ilona Sárvári HorváthGunnar Lidén
Mar 15, 2012·PLoS Computational Biology·Stefan J JolMatthias Heinemann
Apr 27, 2012·PLoS Computational Biology·Rogier Braakman, Eric Smith
May 20, 2014·Enzyme and Microbial Technology·Susann BarigK-Peter Stahmann
Jun 23, 2000·Enzyme and Microbial Technology·M Farfán, I L Calderón
Jun 5, 2015·Current Genetics·Boredi S ChidiFlorian F Bauer
May 12, 2007·FEBS Letters·Maria Concetta PalumboLorenzo Farina
Jul 17, 2014·Microbial Cell Factories·Zhenquan LinXueming Zhao
Apr 29, 2015·Journal of Bioscience and Bioengineering·Kenshi HayakawaHiroshi Shimizu
Jan 8, 2009·Environmental Microbiology·H James TrippStephen J Giovannoni
Jun 14, 2005·Molecular Microbiology·Mohan SubramanianAndrew L Bognar
Jan 31, 2015·Journal of Bioscience and Bioengineering·Shuichi KajihataHiroshi Shimizu
Apr 9, 2015·Applied Microbiology and Biotechnology·Jaco FrankenFlorian F Bauer
Mar 20, 2015·Molecular BioSystems·Xueqin GuYunpeng Qi
Jan 25, 2017·Applied and Environmental Microbiology·Lucie CrépinCarole Camarasa
Mar 25, 2017·Microbial Cell Factories·Nora WeberHarald Heider
Feb 15, 2000·The Journal of Biological Chemistry·Y Q Cheng, J D Walton
Jan 15, 2003·The Journal of Biological Chemistry·Eva AlbersLena Gustafsson
Mar 18, 2009·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Karin StrijbisBen Distel
Aug 21, 2020·Proceedings of the National Academy of Sciences of the United States of America·Johan BjörkerothJens Nielsen

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