PMID: 11316369Apr 24, 2001Paper

Identification of salivary Lactobacillus rhamnosus species by DNA profiling and a specific probe

Research in Microbiology
B RichardA Lonvaud-Funel

Abstract

The Lactobacillus genus has been shown to be associated with the dental carious process, but little is known about the species related to the decay, although Lactobacillus rhamnosus is suspected to be the most implicated species. Conventional identification methods based on biochemical criteria lead to ambiguous results, since the Lactobacillus species found in saliva are phenotypically close. To clarify the role of this genus in the evolution of carious disease, this work aimed to find a rapid and reliable method for identifying the L. rhamnosus species. Methods based on hybridization with DNA probes and DNA amplification by PCR were used. The dominant salivary Lactobacillus species (reference strains from the ATCC) were selected for this purpose as well as some wild strains isolated from children's saliva. DNA profiling using semirandom polymorphic DNA amplification (semi-RAPD) generated specific patterns for L. rhamnosus ATCC 7469. The profiles of all L. rhamnosus strains tested were similar and could be grouped; these strains shared four common fragments. Wild strains first identified with classic methods shared common patterns with the L. rhamnosus species and could be reclassified. One fragment of the profile was purified...Continue Reading

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Citations

Dec 31, 2003·International Journal of Food Microbiology·L Marilley, M G Casey
Oct 20, 2005·Journal of Bioscience and Bioengineering·Katsumi DoiSeiya Ogata
Mar 1, 2004·Journal of Applied Oral Science : Revista FOB·Carlos Ferreira Dos SantosAndrew Seth Greene
Feb 17, 2005·Journal of Medical Microbiology·E M DicksonL Macpherson
Feb 22, 2007·The Journal of Nutrition·Kaouther Ben AmorWillem M de Vos

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