Identification of salt-responsive genes from C4 halophyte Suaeda nudiflora through suppression subtractive hybridization and expression analysis under individual and combined treatment of salt and elevated carbon dioxide conditions

Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology
Saranya JothiramshekarAjay Parida

Abstract

Salinization of soil is a prime abiotic stress that limits agriculture productivity worldwide. To Study the mechanisms that halophytes take up to survive under high salt condition is important in engineering salinity stress tolerance in sensitive species. Suaeda nudiflora is a halophyte plant that grows in the saline environment and extreme high tidal belt. The species have high capability to produce high protein biomass in salty soils due to C4 photosynthesis. The physiological and biochemical changes in S. nudiflora under salinity stress were studied by measuring chlorophyll content, electrolytic leakage, level of lipid peroxidation and total soluble sugars. Increased lipid peroxidation and electrolytic leakage was observed in salt stressed S. nudiflora compared to control plants. A suppression subtractive hybridization strategy was employed to identify differentially expressed genes under salt treatment in S. nudiflora. A total of 333 positive clones were identified and screened. Of these, 250 expressed sequence tags were identified. cDNA subtraction library resulted in 33 contigs and 138 singletons. The functional annotation and metabolic pathways identification were performed using the Blast2GO program. In addition, we ana...Continue Reading

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Datasets Mentioned

BETA
JZ896709
JZ896958

Methods Mentioned

BETA
electrophoresis
PCR
MDA
salt treatment
environmental stresses
transgenic

Software Mentioned

BLAST2GO ( B2GO )
Blast
BlastN
CAP3 Sequence Assembly
Blast2GO
B2GO
Blast2GO InterPro Scan

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