Identification of scavenger receptor B1 as the airway microfold cell receptor for Mycobacterium tuberculosis.

ELife
Haaris S KhanMichael U Shiloh

Abstract

Mycobacterium tuberculosis (Mtb) can enter the body through multiple routes, including via specialized transcytotic cells called microfold cells (M cell). However, the mechanistic basis for M cell entry remains undefined. Here, we show that M cell transcytosis depends on the Mtb Type VII secretion machine and its major virulence factor EsxA. We identify scavenger receptor B1 (SR-B1) as an EsxA receptor on airway M cells. SR-B1 is required for Mtb binding to and translocation across M cells in mouse and human tissue. Together, our data demonstrate a previously undescribed role for Mtb EsxA in mucosal invasion and identify SR-B1 as the airway M cell receptor for Mtb.

References

Jan 1, 1994·American Journal of Respiratory Cell and Molecular Biology·A L CozensD C Gruenert
Jan 23, 1999·Infection and Immunity·P J GiannascaM R Neutra
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·B TrigattiM Krieger
Jun 22, 2000·Pathobiology : Journal of Immunopathology, Molecular and Cellular Biology·T Tschernig, R Pabst
Aug 5, 2000·Virchows Archiv : an International Journal of Pathology·Y Fujimura
Apr 25, 2003·International Journal of Pharmaceutics·Ben ForbesAlison B Lansley
Aug 21, 2003·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hae-Sun ParkP Patrick Cleary
Oct 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sarah A StanleyJeffery S Cox
Dec 31, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Zhenhua YangJoseph H Bates
Feb 5, 2004·Genome Biology·Stefan KurtzSteven L Salzberg
Dec 22, 2005·Infection and Immunity·Peter TyrerJennelle M Kyd
May 27, 2006·Virus Research·Tae-Hwe HeoChang-Yuil Kang
Oct 9, 2007·Nature Reviews. Microbiology·Abdallah M AbdallahWilbert Bitter
Nov 7, 2007·The Journal of Experimental Medicine·Tomonori NochiHiroshi Kiyono
Dec 18, 2007·FEMS Immunology and Medical Microbiology·Sinead C CorrColin Hill
Sep 5, 2008·Current Protocols in Microbiology·Michelle H LarsenWilliam R Jacobs
Dec 17, 2008·Mucosal Immunology·P BrandtzaegM W Russell
May 20, 2009·Bioinformatics·Heng Li, Richard Durbin
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Nov 13, 2009·Molecular Microbiology·Arvind G KinhikarSuman Laal
Nov 16, 2010·Future Microbiology·Meghan E FeltcherMiriam Braunstein
Mar 2, 2011·The Journal of Immunology : Official Journal of the American Association of Immunologists·Dong-Young KimHiroshi Kiyono
Aug 26, 2011·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Jose-Mario FontanillaC Fordham von Reyn
Sep 3, 2011·American Journal of Respiratory Cell and Molecular Biology·Sylwia WilkoszY C Gary Lee
Apr 18, 2012·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·Anton BankevichPavel A Pevzner
Apr 27, 2012·Journal of Bacteriology·Tohru Miyoshi-AkiyamaTeruo Kirikae
Jul 10, 2012·The Journal of Immunology : Official Journal of the American Association of Immunologists·Gaku NakatoHiroshi Ohno
Dec 12, 2012·Human Molecular Genetics·Nicolás Guillermo SantanderDolores Busso
Apr 4, 2014·Journal of Virology·Mariam B Gonzalez-HernandezChristiane E Wobus
Aug 12, 2014·Journal of Proteome Research·Matthew M ChampionPatricia A DiGiuseppe Champion

❮ Previous
Next ❯

Citations

Feb 21, 2021·Mucosal Immunology·Jordan D Davis, Tomasz P Wypych
May 28, 2021·Nature Reviews. Microbiology·Angel Rivera-CalzadaSebastian Geibel

❮ Previous
Next ❯

Datasets Mentioned

BETA
PRJNA605439

Methods Mentioned

BETA
confocal microscopy
flow cytometry
co-immunoprecipitation
immunoprecipitation
affinity purification
PCR
genotyping
FACS

Software Mentioned

GraphPad Prism
GATK
Genome Analysis Toolkit ( GATK
ImageJ
SPAdes
MUMmer
SAMtools
custom Perl script
FlowJo

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.