PMID: 2121742Nov 1, 1990Paper

Identification of sea urchin sperm adenylate cyclase

The Journal of Cell Biology
L H BookbinderV D Vacquier

Abstract

Calmodulin (CaM) affinity chromatography of a detergent extract of sea urchin sperm yielded approximately 20 major proteins. One of these proteins, of Mr 190,000, was purified and used to immunize rabbits. After absorption with living sperm, the serum reacted monospecifically on one- and two-dimensional Western immunoblots with the Mr 190,000 protein. The anti-190-kD serum inhibited 94% of the adenylate cyclase (AC) activity of the CaM eluate. An immunoaffinity column removed 95% of the AC activity, and the purified (but inactive) Mr 190,000 protein was eluted from the column. The antiserum also inhibited 23% of the activity of bovine brain CaM-sensitive AC and 90% of the activity of horse sperm CaM-sensitive AC. These data support the hypothesis that the Mr 190,000 protein is sea urchin sperm AC. Although this AC bound to CaM, it was not possible to demonstrate directly a Ca2+ or CaM sensitivity. However, two CaM antagonists, calmidazolium and chlorpromazine, both inhibited AC activity, and the inhibition was released by added CaM, suggesting the possibility of regulation of this AC by CaM. Indirect immunofluorescence showed the Mr 190,000 protein to be highly concentrated on only the proximal half of the sea urchin sperm flag...Continue Reading

References

Feb 1, 1990·Trends in Biochemical Sciences·K T O'Neil, W F DeGrado
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Citations

Apr 29, 1998·Microscopy Research and Technique·P A Richards, P D Richards
May 10, 2005·Biology of Reproduction·George G Ignotz, Susan S Suarez
Apr 2, 2004·Reproduction : the Official Journal of the Society for the Study of Fertility·Anna T Neill, Victor D Vacquier
May 23, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dan WangDavid L Garbers
May 26, 2007·Biochemical and Biophysical Research Communications·Carmen BeltránAlberto Darszon
Jul 30, 2014·Biochimica Et Biophysica Acta·Victor D VacquierCarmen Beltrán
Jul 20, 2004·Cell Motility and the Cytoskeleton·Mamoru NomuraMasaaki Morisawa
Dec 17, 2005·Journal of Cellular Physiology·Herath Jayantha GunaratneVictor D Vacquier
Jul 19, 2006·Cell Motility and the Cytoskeleton·Mamoru Nomura, Victor D Vacquier
Apr 19, 2006·Journal of Cellular Physiology·U B KauppI Weyand
Jun 28, 2005·Gene·Mamoru NomuraVictor D Vacquier
Feb 4, 2003·Nature Cell Biology·U Benjamin KauppIngo Weyand
Feb 28, 2014·The Journal of Experimental Biology·Martin TresguerresJinae N Roa
Jun 11, 1996·Biochemistry·C BeltránA Darszon

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