PMID: 8955322Dec 1, 1996Paper

Identification of sequences necessary for transcription in vitro from the Chlamydia trachomatis rRNA P1 promoter

Journal of Bacteriology
M Tan, J N Engel

Abstract

Chlamydia trachomatis RNA polymerase was partially purified by heparin-agarose chromatography and used in conjunction with a plasmid-borne G-less cassette template to characterize the C. trachomatis rRNA P1 promoter in vitro. Stepwise mutational analysis revealed that sequences in the -10, -25, and -35 regions are necessary for promoter activity, but no sequence upstream of position -40 is required. Partially purified C. trachomatis RNA polymerase and purified Escherichia coli holoenzyme exhibited some differences in promoter specificity.

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Citations

Nov 18, 2003·Molecular Microbiology·Hilda Hiu Yin Yu, Ming Tan
Nov 6, 2010·Nucleic Acids Research·Yasser M AbdelrahmanRobert J Belland
Jul 5, 2011·Journal of Bacteriology·Elizabeth Di Russo CaseMing Tan
Oct 3, 2012·Proceedings of the National Academy of Sciences of the United States of America·Xiaofeng BaoHuizhou Fan
May 20, 2004·Journal of Bacteriology·Adam C Wilson, Ming Tan
Oct 24, 2006·Journal of Bacteriology·P Scott Hefty, Richard S Stephens
Aug 29, 2006·Journal of Bacteriology·Li ShenYou-Xun Zhang
Nov 12, 2002·Journal of Bacteriology·Adam C Wilson, Ming Tan
Jan 24, 2006·Journal of Bacteriology·Chris S Schaumburg, Ming Tan
Jul 29, 2008·Journal of Bacteriology·Eike NiehusMing Tan
Aug 26, 2000·Journal of Bacteriology·C S Schaumburg, M Tan

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