Identification of serine 205 as a site of phosphorylation on Pax3 in proliferating but not differentiating primary myoblasts.

Protein Science : a Publication of the Protein Society
Patrick J MillerAndrew D Hollenbach

Abstract

Pax3, a member of the paired class homeodomain family of transcription factors, is essential for early skeletal muscle development. Previously, others and we have shown that the stability of Pax3 is regulated on a post-translational level. Evidence in the literature and from our laboratory suggests that phosphorylation, a common form of regulation, may play a role. However, at present, the sites of Pax3 phosphorylation are not known. We demonstrate here the first evidence that Pax3 exists as a phosphoprotein in proliferating mouse primary myoblasts. Using an in vitro kinase assay, deletion, and point mutant analysis, we conclusively identify Ser205 as a site of phosphorylation. The phosphorylation of Ser205 on endogenously expressed Pax3 was confirmed in vivo using antibodies specific for phosphorylation at Ser205. Finally, we demonstrate for the first time that the phosphorylation status of endogenous Pax3 changes rapidly upon the induction of myogenic differentiation. The presence of phosphorylation in a region of Pax3 important for mediating protein-protein interactions, and the fact that phosphorylation is lost upon induction of differentiation, allow for speculation on the biological relevance of phosphorylation.

References

Aug 7, 1992·Cell·T Hunter, M Karin
Apr 30, 1996·Proceedings of the National Academy of Sciences of the United States of America·J A EpsteinR L Maas
Jan 1, 1997·Methods in Cell Biology·T A Rando, H M Blau
Jul 7, 1999·The EMBO Journal·A D HollenbachG Grosveld
Jan 15, 2000·Current Topics in Developmental Biology·S Tajbakhsh, M Buckingham
May 9, 2002·Biochimica Et Biophysica Acta·Andrew D HollenbachGerard Grosveld
Oct 11, 2005·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Shujuan J Xia, Frederic G Barr
May 18, 2007·Annual Review of Cell and Developmental Biology·Margaret Buckingham, Frédéric Relaix
Aug 19, 2007·Biochimica Et Biophysica Acta·Patrick J Miller, Andrew D Hollenbach

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Citations

Dec 21, 2010·Molecular Biology of the Cell·Shunsuke IchiC S K Mayanil
Nov 6, 2008·Pigment Cell & Melanoma Research·Jennifer D KubicDeborah Lang
Jun 9, 2012·Molecular Cancer Research : MCR·Jennifer D KubicDeborah Lang
Oct 13, 2012·Biochemical and Biophysical Research Communications·Aditi S IyengarAndrew D Hollenbach
Feb 15, 2014·Developmental Cell·Margaret Buckingham, Peter W J Rigby
Mar 31, 2017·Biomedical Reports·Claudia Götz, Mathias Montenarh
Jul 4, 2019·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Valentina SalizzatoArianna Donella-Deana
Feb 6, 2020·International Journal of Molecular Sciences·Teresita Padilla-BenavidesAnthony N Imbalzano

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