Identification of Serum miRNA-423-5p Expression Signature in Somatotroph Adenomas

International Journal of Endocrinology
Sida ZhaoYa-Zhuo Zhang

Abstract

Circulating miRNAs are novel disease biomarkers that are valuable for diagnosis and prognosis. But the circulating miRNAs profile in somatotroph adenomas is still unknown. Therefore, serum exosomal miRNAs expression profiling in somatotroph adenomas was performed on 6 somatotroph adenomas and 6 normal controls. From the exosomal miRNAs expression profiling, we found 169 miRNAs differently expressed between somatotroph adenomas and healthy pituitary samples (p< 0.05, FC > 2). Among the 169 miRNAs, miR-423-5p was expressed lower in somatotroph adenomas than in healthy pituitary samples, which was proved by miRSCan Panel Chip™ qPCR. PTTG1 and SYT1 were the target mRNAs of miR-423-5p, and transcriptomics and proteomics profile both indicated the high expression of PTTG1 and SYT1 in somatotroph adenomas. H-scores were 223.1 ± 34.7 for PTTG1 and 163.4 ± 42.3 for SYT1 in 62 somatotroph adenomas specimens and 84.2 ± 21.3 for PTTG1 and 47.4 ± 17.2 for SYT1 in 6 healthy pituitary specimens by IHC. miR-423-5p inhibited the expression of SYT1 and PTTG1 at the mRNA and protein levels. Dual luciferase reporter gene assay shown was significantly reduced in the presence of miR-423-5p in GH3 cells transfected with wild-type PTTG1 3'UTR lucifera...Continue Reading

References

Jun 24, 2004·Nature Reviews. Genetics·Lin He, Gregory J Hannon
Jul 1, 2006·Clinical Medicine & Research·Jacob Tfelt-HansenNaibedya Chattopadhyay
Feb 28, 2007·Endocrine Reviews·George VlotidesShlomo Melmed
Jun 4, 2008·Biological & Pharmaceutical Bulletin·Yuko OgawaRyohei Yanoshita
Jul 1, 2008·Gynecologic Oncology·Douglas D Taylor, Cicek Gercel-Taylor
Jan 21, 2010·Endocrine Reviews·Mark SherlockPaul M Stewart
May 9, 2012·The Journal of Clinical Endocrinology and Metabolism·Daniela D'AngeloAlfredo Fusco
Sep 4, 2013·Journal of Dermatological Science·Jae Cheal YooTae-Jin Yoon
May 30, 2014·Asian Pacific Journal of Cancer Prevention : APJCP·Xiang-Dong XuChun-Li Luo
Jun 14, 2014·Carcinogenesis·Olga VaksmanReuven Reich
Jul 30, 2014·Molecular and Cellular Endocrinology·Zhe Bao WuWei Guo Zhao
Mar 18, 2015·Molecular Medicine Reports·Peng LuoGang Li
Dec 25, 2015·European Journal of Endocrinology·Cristina L RonchiMartin Fassnacht
Nov 1, 2017·Scientific Reports·Saeideh EbrahimkhaniMichael E Buckland
Jan 18, 2018·European Journal of Endocrinology·Leandro KasukiMônica R Gadelha
Mar 21, 2018·Molecular and Cellular Endocrinology·Wang SongZhang Yazhuo
Jul 28, 2018·Translational Lung Cancer Research·Samir M HanashJohannes F Fahrmann
Jul 30, 2018·Translational Research : the Journal of Laboratory and Clinical Medicine·Ying-Hsiu SuSurbhi Jain
Aug 9, 2018·Endocrine, Metabolic & Immune Disorders Drug Targets·Franca DicuonzoVicenzo Triggiani

❮ Previous
Next ❯

Citations

Mar 24, 2020·Endocrine Reviews·Vinaya Srirangam Nadhamuni, Márta Korbonits
Jun 6, 2020·Biomedicines·Wilhelm GossingLars Radke
Oct 8, 2020·International Journal of Molecular Sciences·Ozal BeylerliGjumrakch Aliev
Oct 18, 2020·International Journal of Molecular Sciences·Simone DonatiMaria Luisa Brandi
Feb 13, 2021·Frontiers in Endocrinology·Mengqi ChangRenzhi Wang

❮ Previous
Next ❯

Methods Mentioned

BETA
biopsy
proteomics profiling
transmission electron microscopy
Chip
ELISA
flow cytometry
protein assay
electrophoresis
Assay
PCR

Software Mentioned

Targetscan
ProteinPilotTM
SPSS Statistics
ImageStream
Mascot
ImageJ
Ensembl
miRanda
miRSCan

Related Concepts

Related Feeds

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.

Cell Migration

Cell migration is involved in a variety of physiological and pathological processes such as embryonic development, cancer metastasis, blood vessel formation and remoulding, tissue regeneration, immune surveillance and inflammation. Here is the latest research.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis