Identification of serum protein biomarkers in biliary atresia by mass spectrometry and enzyme-linked immunosorbent assay

Journal of Pediatric Gastroenterology and Nutrition
Zai SongShan Zheng

Abstract

There is an urgent need to identify noninvasive and convenient biomarkers for early diagnosis of biliary atresia (BA). The aim of the present study was to identify potential protein biomarkers for BA. Serum samples from 42 infants with BA, 38 infants with non-BA neonatal cholestasis (NC), and 36 healthy controls (HC) were randomly divided into a training set and a test set. Serum proteomic profiles were measured using surface-enhanced desorption/ionization time-of-flight mass spectrometry. Candidate biomarkers were purified using high-performance liquid chromatography, identified using liquid chromatography tandem mass spectrometry, and validated using enzyme-linked immunosorbent assay. A total of 2 protein peaks (m/z with 8697 and 9098  Da) with differential expression levels were found using surface-enhanced desorption/ionization time-of-flight mass spectrometry. These peaks were then analyzed by a support vector machine to construct a classification model in the training set. The sensitivity and specificity of the model were 94.1% and 91.8%, respectively, in the test set. One candidate biomarker (9098  Da) was identified as Apo C-II, and was found to be downregulated in BA samples compared with HC samples, and upregulated in...Continue Reading

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Citations

Jan 11, 2013·ISRN Surgery·Barbara E Wildhaber
Oct 10, 2015·Journal of Proteome Research·Kejun ZhouWei Jia
Jul 3, 2015·Frontiers in Pediatrics·Thomas GötzeAndré Hoerning
Apr 23, 2015·Journal of Proteome Research·Kejun ZhouWei Cai
Jul 5, 2019·Journal of Gastroenterology and Hepatology·Junfeng WangRui Dong
Nov 24, 2017·Science Translational Medicine·Chatmanee LertudomphonwanitJorge A Bezerra
Jul 12, 2017·Gastroenterology Research and Practice·Rui DongShan Zheng
Jan 14, 2017·World Journal of Hepatology·Krishna Kumar Govindarajan
May 26, 2017·Clinical Liver Disease·Daniel H Leung
Mar 4, 2021·Scientific Reports·Ken-Ichiro KonishiAkihiko Kimura

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