PMID: 9185565Jul 1, 1997Paper

Identification of sigma factors for growth phase-related promoter selectivity of RNA polymerases from Streptomyces coelicolor A3(2)

Nucleic Acids Research
J G KangJung-Hye Roe

Abstract

We examined the promoter selectivity of RNA polymerase (RNAP) from Streptomyces coelicolor at two growth phases by in vitro transcription. Distinct sets of promoters were preferentially recognized by either exponential or stationary phase RNAP. No change in molecular weight or net charge of the core subunits was observed, suggesting that the associated specificity factors determined phase-specific promoter selectivity of the holoenzyme. Five different specificity factors and their cognate promoters were identified by in vitro holoenzyme reconstitution and transcription assays. sigma66 (sigma hrdB) and sigma46 (sigma hrdD) recognized promoters (rrnD p2 and dagA p4 for sigma66, actII-orf4 p and whiB p2 for sigma46) preferentially transcribed by the exponential phase RNAP. sigma52 recognized promoters (dagA p3 and actIII px1) preferentially transcribed by the stationary phase RNAP. Sigma28 (sigma sigE) recognized promoters (hrdD p1, whiB p1 and dagA p2) transcribed equally by both RNAPs. A novel 31 kDa specificity factor recognized actIII px2, glnR p2 and hrdD p2 promoters preferentially transcribed by the stationary phase RNAP. This factor was isolated from the stationary phase RNAP and reconstituted holoenzyme in vitro as a sigm...Continue Reading

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Citations

Apr 12, 2005·Applied Microbiology and Biotechnology·Rosa AlduinaAnna Maria Puglia
Jan 19, 2011·Applied Microbiology and Biotechnology·Nicolas SeghezziMarie-Joëlle Virolle
Jan 14, 2011·Applied Microbiology and Biotechnology·Yvonne TiffertYvonne Mast
May 9, 2001·Biochimica Et Biophysica Acta·P Bralley, G H Jones
Jun 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·Ying ZhuoLi-Xin Zhang
Jan 31, 2009·Antioxidants & Redox Signaling·Youngkyun KimKwangseog Ahn
May 21, 2013·Applied and Environmental Microbiology·Weishan WangKeqian Yang
May 15, 2007·Journal of Bacteriology·Gavin A ByrneWim G Meijer
Dec 13, 2005·Microbiology and Molecular Biology Reviews : MMBR·Mark J KazmierczakKathryn J Boor
Dec 18, 2008·BMC Genomics·Yoon Jung KimYong Keun Chang
Jun 8, 2010·BMC Microbiology·Carla Th P HopmanYvonne Pannekoek
Aug 9, 2011·Bioscience, Biotechnology, and Biochemistry·Lingzhu MengJoo-Won Suh
May 27, 2014·Applied Microbiology and Biotechnology·Karel MikulíkJurgen Felsberg
Dec 14, 2011·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Hiroki EtohTakuo Akimoto
Jan 19, 2016·Biochemical and Biophysical Research Communications·Keon Young KimSangYoun Park
Mar 12, 2008·Physiologia Plantarum·Takashi OsanaiKan Tanaka
Jun 15, 2007·IEEE Transactions on Pattern Analysis and Machine Intelligence·Gozde UnalGreg Slabaugh

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