PMID: 8938982Jan 1, 1996Paper

Identification of single and dual infections with distinct subtypes of human immunodeficiency virus type 1 by using restriction fragment length polymorphism analysis

Virus Genes
L M JaniniM A Rayfield

Abstract

The simultaneous presence of multiple HIV-1 subtypes has become common in communities with the growth of the pandemic. As a consequence, the potentiality for an increased frequency of HIV-1 mixed infections caused by viruses of distinct subtypes could be expected. Thus, there is a need to estimate the prevalence and geographic distribution of infections caused by viruses of a singular subtype as well as coinfections caused by two or more HIV-1 strains of distinct subtypes. To address this need, we have developed a genetic method based on restriction fragment length polymorphism (RFLP) to screen for these two types of infections within infected populations. In this assay, restriction enzymes may be used to predict the phylogroup of HIV-1 infected samples. A 297 bp pol fragment spanning the entire viral protease gene and a 311 bp fragment of the p24 gag region are used for this analysis. The viral regions are amplified by nested PCR using DNA templates from uncultured peripheral blood mononuclear cells (PBMC) or virus culture. Classification of HIV-1 strains to well defined subtypes B, D, F, and A/C is done by sequential endonuclease restriction analysis of a PCR amplified-protease gene followed by analysis of the p24 gag region....Continue Reading

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Citations

Nov 12, 2003·The Journal of Allergy and Clinical Immunology·Todd M Allen, Marcus Altfeld
May 12, 2004·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Chunfu YangRenu B Lal
May 30, 2001·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·M L GuimarãesM G Morgado
Apr 20, 2000·AIDS Research and Human Retroviruses·A HolguínV Soriano
Oct 6, 2000·AIDS Research and Human Retroviruses·A HolguínV Soriano
Dec 19, 2002·AIDS Research and Human Retroviruses·Monick Lindenmeyer GuimarãesUNKNOWN Brazilian Network for HIV Isolation and Characterization
Jun 21, 2003·AIDS Research and Human Retroviruses·Marquita V GittensPaul N Levett
Apr 30, 1999·AIDS Research and Human Retroviruses·D PieniazekM A Rayfield
Feb 24, 1999·AIDS Research and Human Retroviruses·J L MellquistS H Eshleman
Jan 25, 2005·AIDS Research and Human Retroviruses·Clement ZehMarcia L Kalish
Jun 10, 2005·AIDS Research and Human Retroviruses·Tamara L SchroederSean M Philpott
Jul 13, 2006·AIDS Research and Human Retroviruses·Karl-Heinz HerbingerMichael Hoelscher
Nov 13, 2008·AIDS Research and Human Retroviruses·Carlos Augusto YabarEberth Quijano
Aug 26, 1998·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·M G MorgadoV Bongertz
Feb 3, 1999·Journal of Acquired Immune Deficiency Syndromes and Human Retrovirology : Official Publication of the International Retrovirology Association·A TanuriM Rayfield
Oct 2, 2012·Virology Journal·Ana Carolina Soares de OliveiraSabri Saeed Sanabani
Mar 22, 2014·PloS One·Peter N FonjungoDanuta Pieniazek
Dec 29, 1998·Emerging Infectious Diseases·D PieniazekW Heneine
Mar 19, 1999·Emerging Infectious Diseases·A RamosD Pieniazek
Mar 11, 2003·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Erika CastroMariza G Morgado
Jun 12, 2008·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Jean-Marie ZimmerMarc De Maeyer
Jun 24, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·J N NkengasongM Rayfield
Jun 3, 2000·Journal of Acquired Immune Deficiency Syndromes : JAIDS·A C VicenteA Tanuri
Apr 22, 2006·Journal of Medical Virology·Sylvia Lopes Maia TeixeiraMariza Gonçalves Morgado

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