Identification of six new polymorphisms in the human coronavirus 229E receptor gene (aminopeptidase N/CD13)

International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases
Leen VijgenMarc Van Ranst

Abstract

Human aminopeptidase N (APN/CD13/ANPEP) has been identified as the receptor for human coronavirus (HCoV) 229E. In this study, we analyzed the region of the APN gene that encodes a stretch of amino acid residues, essential for its HCoV-229E receptor function (amino acids 260-353). Full-length APN exon 3, intron 3 and exon 4, was PCR-amplified and sequenced in DNA samples from 100 unrelated Caucasian Belgian healthy volunteers. We identified seven polymorphisms, including four intron 3 and three exon 4 variations. Apart from the already known C956T exon 4 mutation, the six other polymorphisms have not yet been described. The most prevalent APN variations in this population (C956T leading to an alanine to valine substitution, G978T, G987A and intron3-C389T) always occurred together at an allele frequency of 8.5%. Haploid DNA sequencing demonstrated the presence of these four variations on the same allele. Three polymorphisms in intron 3, intron3-G395C, intron3-C86T, and intron3-C429T, were identified with an allele frequency of 3.5%, 1% and 0.5% respectively. Five haplotypes were identified in the population of 100 individuals. These results demonstrate that there is a relatively broad spectrum of variations in the APN domain crit...Continue Reading

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Citations

Jun 2, 2005·Proceedings of the National Academy of Sciences of the United States of America·Ralph S Baric, Amy C Sims
Feb 13, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Diane BimczokHermann-Josef Rothkötter
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Jun 18, 2019·BioMed Research International·Ana Cecilia Lorenzo-LealHoracio Bach
Dec 19, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Chenyang LuDavid A Fox

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Datasets Mentioned

BETA
X13276
AC018988
AJ421875
AF527789

Methods Mentioned

BETA
PCR

Software Mentioned

BLAST ( Basic Local Alignment Search Tool )
SeqMan
LaserGene

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