Abstract
The cell-based assay using yeast deletion mutants has been recognized as an efficient analysis to discover therapeutic compounds and reveal their mode of action. In this study, S. pombe deletion mutants-based HTS screening was carried out to identify potential anti-cancer agents. The NCI chemical library of 5700 compounds was screened using kit strains, which consisted of S. pombe mutants harboring deletions in genes involved in DNA repair and mitotic control. During the screening, we identified 40 compounds conferring growth inhibition of S. pombe. Their anti-tumorigenic properties were examined by phenotypic effect on S. pombe, flow cytometry and apoptosis analysis of human cancer. Here, we report hit compounds inducing apoptosis for development of anti-cancer agents suggesting that S. pombe deletion mutants are useful in identifying potential anti-cancer agents in human cancer therapeutics.
References
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
May 1, 1994·Nature Genetics·E Fisher, P Scambler
Jun 1, 1996·Journal of Bacteriology·M L Dickens, W R Strohl
Aug 8, 1997·The Journal of Biological Chemistry·Y J JangH S Yoo
Mar 18, 1999·Nature Genetics·G GiaeverR W Davis
Mar 29, 2001·Planta medica·W N SetzerW A Haber
May 18, 2001·BioTechniques·J R LambJ A Simon
Jul 20, 2001·The Journal of Biological Chemistry·K S ChungH S Yoo
Dec 14, 2001·Biochemical and Biophysical Research Communications·M WonH S Yoo
Jan 17, 2002·Journal of the National Cancer Institute·Heather M DunstanJohn R Lamb
Feb 23, 2002·Nature·V WoodL Cerrutti
Sep 4, 2003·The Journal of Biological Chemistry·Jun WangSheo Singh
Dec 9, 2003·Nature Biotechnology·Ainslie B ParsonsCharles Boone
Jan 14, 2004·Cell·Pek Yee LumDaniel D Shoemaker
May 12, 2004·FEBS Letters·Jung Kyoon ChoiSangsoo Kim
Jul 29, 2004·Phytochemistry·Alex Haroldo JellerMaysa Furlan
Citations
Oct 15, 2013·Cancer Chemotherapy and Pharmacology·Kyung-Sook ChungMisun Won
May 1, 2010·Investigational New Drugs·Bo-Kyung KimMisun Won
Nov 19, 2010·Carcinogenesis·Bo-Kyung KimMisun Won
Oct 3, 2013·Apoptosis : an International Journal on Programmed Cell Death·Kyoung-Jae WonMisun Won
Nov 12, 2013·International Journal of Cancer. Journal International Du Cancer·Kyoung-Jae WonMisun Won
Apr 28, 2010·PLoS Genetics·Mirela MatecicJeffrey S Smith
Jan 24, 2014·American Journal of Physiology. Endocrinology and Metabolism·Yun Chau LongBor Luen Tang
Sep 19, 2014·International Journal of Oncology·Soma KunduHyung Sik Kim
Oct 29, 2019·DNA and Cell Biology·Merve YılmazerBedia Palabıyık