Identification of small noncoding RNAs in Helicobacter pylori by a bioinformatics-based approach.

Current Microbiology
Bin XiaoQuan-Ming Zou

Abstract

Small noncoding RNAs (sRNAs) are a group of regulatory RNA molecules normally without a protein-coding function. In recent years, the importance of sRNAs as mediators of gene expression in bacteria has begun to be recognized. More than 70 sRNAs have been known in Escherichia coli. However, little is known about sRNAs in Helicobacter pylori, a human pathogen associated with gastric diseases. Here, we systematically identified sRNAs in the H. pylori genome by a computational approach based on gene location, sequence conservation, promoter and terminator search, and secondary structure. Among a total of six candidate sRNAs initially predicted, two novel sRNAs (IG-443 and IG-524) were confirmed by Northern blot and reverse transcription-polymerase chain reaction (RT-PCR). Virtually, they were a class of natural antisense transcripts, which were complementary to partial sequences of the following genes: flagellar motor switch gene (fliM) and fumarase (fumC). Taken together, the results indicate that there exist novel sRNAs in H. pylori and these RNAs might play a potential role in regulating gene expression.

References

Jan 1, 1994·Annual Review of Microbiology·E G Wagner, R W Simons
Jul 5, 2001·International Journal of Medical Microbiology : IJMM·V ScarlatoD Beier
Jul 11, 2001·Genes & Development·K M WassarmanS Gottesman
Jul 13, 2001·Current Biology : CB·L ArgamanS Altuvia
Sep 28, 2001·Nucleic Acids Research·R J CarterS R Holbrook
Mar 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Eric Massé, Susan Gottesman
May 23, 2002·Journal of Medical Microbiology·Zun-Wu ZhangMichael J G Farthingt
Jun 6, 2002·Trends in Genetics : TIG·E Gerhart H Wagner, Klas Flärdh
Oct 19, 2004·Annual Review of Microbiology·Susan Gottesman
May 26, 2005·Trends in Genetics : TIG·Susan Gottesman
Jul 29, 2005·Nucleic Acids Research·Jonathan LivnyMatthew K Waldor
Dec 20, 2005·Molecular Microbiology·Maude Guillier, Susan Gottesman

❮ Previous
Next ❯

Citations

Jun 24, 2010·The Journal of Microbiology·Shana MilesD Scott Merrell
Apr 15, 2009·Infection and Immunity·Beth M CarpenterD Scott Merrell
Jan 26, 2011·MBio·Yili ChenEleftherios T Papoutsakis
Mar 5, 2011·Microbiology and Molecular Biology Reviews : MMBR·Jeremy J GilbreathDavid R Hendrixson
Feb 2, 2011·BMC Bioinformatics·Alexander Herbig, Kay Nieselt
Sep 27, 2013·PLoS Genetics·Wen Fong OoiPatrick Tan
Aug 17, 2010·Annual Review of Genetics·Maureen Kiley Thomason, Gisela Storz
Dec 1, 2011·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Ruiguang Ge, Xuesong Sun
Jan 24, 2013·Metallomics : Integrated Biometal Science·Amanda G Oglesby-Sherrouse, Erin R Murphy
Dec 22, 2009·Current Opinion in Microbiology·Jane M Liu, Andrew Camilli
Dec 19, 2015·BMC Genomics·Casey L C SchroederSanjeev K Sahni
Jun 29, 2010·FEMS Microbiology Reviews·Alberto Danielli, Vincenzo Scarlato
Apr 28, 2011·RNA Biology·Michael-Paul VockenhuberBeatrix Suess
Jan 1, 2011·Genes·Jan ReinkensmeierAnke Becker
Aug 27, 2015·World Journal of Experimental Medicine·Remo GeorgeM Tino Unlap
Oct 15, 2014·Journal of Bacteriology·Chen-Hsun TsaiLydia M Contreras
Jun 1, 2016·Journal, Genetic Engineering & Biotechnology·Praveen P BalgirPuneet Kaur
Apr 22, 2016·World Journal of Gastroenterology : WJG·Xiao-Jun YangPeng Gao
Oct 26, 2018·Frontiers in Immunology·Martin D KnolleAndrew N J McKenzie
Apr 18, 2019·Biochimie·Maxence LejarsEliane Hajnsdorf
Aug 3, 2021·Frontiers in Microbiology·Alejandro Tejada-Arranz, Hilde De Reuse

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.