Identification of species of the Burkholderia cepacia complex by sequence analysis of the hisA gene

Journal of Medical Microbiology
Maria Cristiana PapaleoPeter Vandamme

Abstract

Bacteria of the Burkholderia cepacia complex (Bcc) are opportunistic human pathogens that can cause serious infections in the lungs of cystic fibrosis patients. The Bcc is a complex taxonomic group and comprises 17 closely related species of both biotechnological and clinical importance that have been discriminated by a polyphasic taxonomic approach. In this study we focused on the hisA gene, which encodes a 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino) methylideneamino] imidazole-4-carboxamide isomerase involved in histidine biosynthesis, as a new target gene to discriminate among the Bcc species. PCR primers were designed to amplify a hisA DNA fragment of 442 bp from 78 strains representative of all the 17 Bcc species known at the time of writing. The nucleotide sequences of the amplicons were determined and aligned with the 54 Bcc sequences available in databases. Then a phylogenetic tree was constructed on the basis of this alignment and this revealed that this hisA region allows discrimination of all Bcc species, suggesting that this gene fragment can be used for the identification of Bcc strains. In addition, an 11 nucleotide letter code for the rapid discrimination of Bcc species was identified.

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Citations

Dec 17, 2015·Letters in Applied Microbiology·M MartinucciE De Gregorio
Apr 27, 2013·New Biotechnology·Maria Cristiana PapaleoRenato Fani
Aug 4, 2015·Journal of Microbiological Methods·Elena PerrinRenato Fani
Jan 25, 2011·Systematic and Applied Microbiology·Peter Vandamme, Peter Dawyndt
Jun 3, 2014·European Journal of Microbiology & Immunology·H FrickmannR M Hagen
Oct 16, 2015·The Open Microbiology Journal·Maria Cristiana PapaleoRenato Fani
Nov 3, 2017·International Journal of Systematic and Evolutionary Microbiology·Pablo MartinaAlejandra Bosch
Apr 17, 2020·Clinical Microbiology Reviews·Mariana TavaresIsabel Sá-Correia
Feb 6, 2019·Annals of Clinical Microbiology and Antimicrobials·Naveen Kumar Devanga Ragupathi, Balaji Veeraraghavan

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