PMID: 9189826Jun 15, 1997Paper

Identification of specific calcium-binding noncollagenous proteins associated with glutaraldehyde-preserved bovine pericardium in the rat subdermal model

Journal of Biomedical Materials Research
T A GuraC L Webb

Abstract

Calcification of glutaraldehyde-preserved bioprosthetic heart valves (BHVs) results in their clinical failure. The mechanism of this pathologic calcification is not well defined. Since serum proteins are known to be taken up in mineralized tissue, we hypothesized that serum proteins derived from several calcium-binding noncollagenous proteins (NCPs) of bone and teeth also may be associated with pathologically mineralized BHVs. Using a rat subdermal model of BHV calcification, glutaraldehyde-preserved bovine pericardium (GPBP) was implanted for 1, 3, 14, and 60 days, and then subjected to an extraction procedure designed to isolate only NCPs tightly bound to the mineral phase. Gel electrophoresis and Coomassie Brilliant Blue staining demonstrated that these proteins became associated with GPBP over time, paralleling reported calcium uptake by the tissue. Stains-All staining demonstrated a marked accumulation of highly acidic, phosphorylated NCPs associated with 60-day GPBP extracts. Some of these proteins were detected in rat serum but were absent from extracts of GPBP incubated in rat serum in vitro. Western blotting with antibodies to three NCPs found in bone and teeth-bone acidic glycoprotein 75 (BAG 75), osteopontin, and SPA...Continue Reading

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Citations

Jan 5, 2002·Journal of Biomedical Materials Research·Kookmin M Kim
Aug 12, 2006·Current Rheumatology Reports·Lauren M Pachman, Adele L Boskey
Jul 1, 1997·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·N R VyavahareR J Levy
Jul 16, 2005·Cardiology·Bharat WarrierChi H Lee
Oct 17, 2002·Rheumatic Diseases Clinics of North America·Lauren M Pachman
Feb 5, 1999·Circulation Research·T WadaC M Giachelli
Mar 18, 1999·The American Journal of Pathology·K M KimH D Battarbee

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