PMID: 9437853Jan 23, 1998Paper

Identification of sperm-bearing female-specific chromosome in the sex-reversed chicken

The Journal of Experimental Zoology
AbinawantoK Shimada

Abstract

Sexual differentiation in the female chick embryo was phenotypically reversed to the male sex by injection of an aromatase inhibitor (Fadrozole, 0.1 mg/egg) into the embryo at day 5 of incubation. The birds were raised to 10 months of age, and the morphology of the gonads of sex-reversed hens were examined by the light-microscopic morphology, and the presence of the W chromosome gene was determined by fluorescence in situ hybridization (FISH) technique and used for PCR analysis of a single isolated sperm. The sex-reversed hens possessed two testes with a fully developed oviduct on the left side. The testes contained essentially the same cellular components as those of normal testes, although sperm counts were low. FISH analysis revealed numerous spermatids and several sperm bearing W-chromosomes, indicating that the second meiosis occurred normally but that the transformation from the spermatid to the spermatozoon is partially impaired. PCR analysis using the DNA of a single sperm also indicates that sperm-carrying the W chromosome were produced.

References

Jan 24, 1992·Science·A Elbrecht, R G Smith
May 1, 1980·General and Comparative Endocrinology·R StollR Maraud
Apr 1, 1995·Biology of Reproduction·Y Kimura, R Yanagimachi
Dec 1, 1995·Molecular Reproduction and Development·H KagamiR J Etches
Mar 1, 1996·The Journal of Biological Chemistry·D EusèbeJ Y Picard
Oct 1, 1996·Biology of Reproduction·S KuretakeR Yanagimachi

❮ Previous
Next ❯

Citations

Mar 21, 2007·Poultry Science·S TakagiK Shimada
Dec 10, 2009·The Journal of Reproduction and Development·Marcos B ValdezTakahiro Yamagata
May 5, 2011·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·X YangN Yang
Aug 7, 2007·Cytogenetic and Genome Research·K ShimadaT Namikawa
Dec 19, 2001·The Journal of Experimental Zoology·S VaillantN Richard-Mercier
Aug 27, 2019·Sexual Development : Genetics, Molecular Biology, Evolution, Endocrinology, Embryology, and Pathology of Sex Determination and Differentiation·Yu-Qiong HuAng Li
Mar 25, 2017·Biology of Reproduction·Chunhai LiuUlrich Wernery
Jan 31, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Hiroshi KagamiTamao Ono
Sep 3, 2021·Veterinary Medicine and Science·Salwan M AbdulateefMohammed Q Al-Ani

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.