Identification of STAG1 as a key mediator of a p53-dependent apoptotic pathway

Oncogene
Yoshio AnazawaYusuke Nakamura

Abstract

A mutant version of p53 (p53-121F), in which phenylalanine replaces the 121st serine residue, can induce apoptosis more effectively than wild-type p53 (wt-p53). In view of this observation, we considered that one or more apoptosis-related p53-target genes might be preferentially induced by p53-121F. We carried out cDNA microarray analysis to identify such genes, using mRNAs isolated from LS174T colon-cancer cells infected by adenovirus vectors containing either p53-121F (Ad-p53-121F) or wt-p53 (Ad-p53). The STAG1 gene was one of the transcripts showing higher expression levels in cells infected with Ad-p53-121F as opposed to Ad-wtp53. The encoded product appears to contain a transmembrane domain, and binding motifs for SH3 and WW. In two other cancer cell lines, the expression of STAG1 mRNA was induced in response to various genotoxic stresses in a p53-dependent manner; moreover, enforced expression of STAG1 led to apoptosis in several additional cancer cell lines. Suppression of endogenous STAG1 using the RNA-interference method reduced the apoptotic response, whether induced by Ad-p53-121F or Ad-p53. These results suggest that STAG1, a novel transcriptional target for p53, mediates p53-dependent apoptosis, and might be a good...Continue Reading

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Citations

Aug 10, 2006·Journal of Human Genetics·Yoshiko MasudaHirofumi Arakawa
Oct 5, 2010·The Journal of Biological Chemistry·Naoko NakanoMitsuyasu Kato
Mar 16, 2006·Nucleic Acids Research·Kentaro YanoTakao Hanashita
Oct 18, 2013·Cancer Medicine·Masahiro TakahashiChikashi Ishioka
Jan 21, 2014·Cancer Science·Thanh Thao Vo NguyenMitsuyasu Kato
Dec 9, 2014·Cell Cycle·Martin FischerKurt Engeland
Mar 12, 2015·Environmental Toxicology and Chemistry·Xiuping ChenYufa Peng
Jun 16, 2015·Nature Genetics·Kumarasamypet M MohankumarRichard J Gilbertson
Jun 27, 2018·Journal of Biochemistry·Susumu Itoh, Fumiko Itoh
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Mar 18, 2020·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Meidi U PuteriMitsuyasu Kato
Jul 31, 2019·International Journal of Molecular Sciences·Bi-He CaiJang-Yi Chen
Nov 26, 2010·Molecular Biology Reports·Dan ZhengYumin Mao

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