PMID: 9428692Jan 15, 1998Paper

Identification of Stat 5B as a substrate of the insulin receptor

European Journal of Biochemistry
D Sawka-VerhelleE Van Obberghen

Abstract

We have screened a human placenta library using the yeast two-hybrid system to identify proteins that interact with the cytoplasmic domain of the insulin receptor. Doing so, we trapped a cDNA clone which encodes the Stat 5B region comprising amino acids 469 to 786. We show that interaction between Stat 5B and the receptor requires a functional insulin-receptor kinase, Tyr960 of insulin receptor is implicated in the interaction with Stat 5B, whereas asparagine and proline forming the NPEY960-motif are not, and Stat 5B mutated at Thr684, a potential phosphorylation site of mitogen-activated protein kinase, loses its ability to interact with the insulin receptor. Further, we found that insulin promotes rapid tyrosine phosphorylation of endogenous Stat 5B in 293 EBNA cells overexpressing insulin receptor and in NHIR cells. Taken together, our findings suggest that Stat 5B corresponds to a substrate for the insulin-receptor kinase, and this widens the repertoire of insulin-signaling pathways.

References

Mar 25, 1992·Nucleic Acids Research·D GietzR H Schiestl
Aug 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·J WhittakerC A Hofmann
Aug 1, 1987·Molecular and Cellular Biology·C Chen, H Okayama
Oct 19, 1995·Nature·J N Ihle
Sep 12, 1995·Proceedings of the National Academy of Sciences of the United States of America·X LiuL Hennighausen
Sep 14, 1995·Nature·X J SunM F White
Sep 1, 1994·Diabetologia·E Van Schaftingen
Mar 8, 1996·The Journal of Biological Chemistry·Y HanG R Stark
Nov 1, 1996·Molecular and Cellular Biology·D WangJ N Ihle
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·O K ParkD Nathans
Mar 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J ChenL H Wang
Jun 27, 1997·The Journal of Biological Chemistry·D Sawka-VerhelleE Van Obberghen
Sep 1, 1996·Trends in Cell Biology·J BriscoeM Müller

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Citations

Dec 12, 2001·European Journal of Clinical Investigation·E Van ObberghenJ Giudicelli
Jan 4, 2008·Acta Physiologica·P Lebrun, E Van Obberghen
Apr 23, 1999·Journal of Basic and Clinical Physiology and Pharmacology·M E Patti, C R Kahn
Oct 12, 2012·Cellular and Molecular Life Sciences : CMLS·Latha RamalingamDebbie C Thurmond
Mar 19, 2014·Protein & Cell·Yipeng Du, Taotao Wei
Feb 27, 2003·Biochemical and Biophysical Research Communications·Sanjin ZvonicRandall L Mynatt
Nov 8, 2005·The Journal of Biological Chemistry·Jie XuJoseph L Messina
May 1, 1999·The Journal of Biological Chemistry·S JiJ L Messina
Sep 25, 1998·The Journal of Biological Chemistry·A Kasus-JacobiA F Burnol
Mar 30, 2001·The Journal of Biological Chemistry·C L SadowskiH B Sadowski
Apr 28, 2001·The Journal of Biological Chemistry·P PeraldiE Van Obberghen
Mar 11, 1999·Journal of Receptor and Signal Transduction Research·M H Heim
May 24, 2000·The Journal of Biological Chemistry·B EmanuelliE Van Obberghen

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