Identification of Strains of Citrus tristeza virus by Subtraction Hybridization

Plant Disease
K S DerrickR Harakava

Abstract

Citrus sudden death (CSD) appears to be a new disease that is a serious problem in Brazil. Symptoms of CSD include yellow stain in the phloem of the rootstock. The cause is not known, but it appears to be infectious and may only affect trees budded on Rangpur lime. In a survey in Brazil, in addition to CSD, we observed numerous trees on Rangpur lime that were obviously declining but had remained in production for several years. Trees with this disease, referred to as Rangpur lime decline (RLD) were different from those with citrus blight (CB). They had near-normal size fruit compared with the small fruit associated with CB and were negative in the serological test for the CB-associated protein (p12). Moreover, they did not have the yellow stain symptom and obviously were declining much more slowly than was reported for CSD. To determine what viruses or virus strains might be associated with CSD, double-stranded (ds)RNAs from fibrous roots of a tree with CSD and stem bark from greenhouse trees infected with Citrus tristeza virus (CTV) isolates T30 and T36 were used to make random primed cDNAs. A Clontech PCR-Select cDNA Subtraction Kit was used to subtract the CSD cDNA with cDNA from an equal mixture of dsRNA from T30 and T36. O...Continue Reading

References

Dec 23, 1998·Plant Molecular Biology·T L CeccardiK S Derrick
Nov 10, 2001·Annual Review of Phytopathology·K S Derrick, L W Timmer
Mar 14, 2002·Plant Physiology·Yi LiSimon J McQueen-Mason
May 10, 2008·Genome Génome / Conseil National De Recherches Canada·Z DengF G Gmitter
Oct 24, 2008·Phytopathology·M E HilfS M Garnsey

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