Identification of structural and molecular determinants of the tyrosine-kinase Wzc and implications in capsular polysaccharide export

Molecular Microbiology
Emmanuelle BéchetChristophe Grangeasse

Abstract

Capsular polysaccharides are well-established virulence factors of pathogenic bacteria. Their biosynthesis and export are regulated within the transmembrane polysaccharide assembly machinery by the autophosphorylation of atypical tyrosine-kinases, named BY-kinases. However, the accurate functioning of these tyrosine-kinases remains unknown. Here, we report the crystal structure of the non-phosphorylated cytoplasmic domain of the tyrosine-kinase Wzc from Escherichia coli in complex with ADP showing that it forms a ring-shaped octamer. Mutational analysis demonstrates that a conserved EX(2) RX(2) R motif involved in subunit interactions is essential for polysaccharide export. We also elucidate the role of a putative internal regulatory tyrosine and we show that BY-kinases from proteobacteria autophosphorylate on their C-terminal tyrosine cluster via a single-step intermolecular mechanism. This structure-function analysis also allows us to demonstrate that two different parts of a conserved basic region called the RK-cluster are essential for polysaccharide export and for kinase activity respectively. Based on these data, we revisit the dichotomy made between BY-kinases from proteobacteria and firmicutes and we propose a unique pr...Continue Reading

References

Nov 1, 1990·Trends in Biochemical Sciences·M SarasteA Wittinghofer
Dec 24, 1997·Psychological Reports·J Gumbiner
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·H R Powell
Dec 26, 2001·The Journal of Biological Chemistry·Christophe GrangeasseAlain J Cozzone
Jan 5, 2002·Applied Microbiology and Biotechnology·D BergmaierC P Champagne
Mar 28, 2002·Journal of Molecular Biology·Detlef D LeipeL Aravind
Jul 26, 2002·The Journal of Biological Chemistry·Patricia DoubletAlain J Cozzone
Oct 24, 2002·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsThomas C Terwilliger
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Oct 22, 2004·Contributions to Microbiology·Clare M Taylor, Ian S Roberts
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·E BlancG Bricogne
Mar 28, 2006·The Journal of Biological Chemistry·Didier SoulatChristophe Grangeasse
Jun 8, 2006·Annual Review of Biochemistry·Chris Whitfield
Oct 6, 2006·Journal of Molecular Biology·Marcus D HartmannHans D Bartunik
Jan 9, 2007·Trends in Biochemical Sciences·Christophe GrangeasseIvan Mijakovic
Jan 27, 2007·Journal of Molecular Biology·Brice ObadiaChristophe Grangeasse
Feb 7, 2007·Proceedings of the National Academy of Sciences of the United States of America·Richard F CollinsJames H Naismith
Jan 22, 2008·Nature Structural & Molecular Biology·Ante TociljMiroslaw Cygler
Dec 9, 2008·Trends in Biochemical Sciences·Renato MoronaMiroslaw Cygler
Aug 1, 2007·Journal of Applied Crystallography·Airlie J McCoyRandy J Read
Jun 16, 2009·Trends in Biochemical Sciences·Daniel C Lee, Zongchao Jia
Sep 1, 2009·Biochimica Et Biophysica Acta·Christophe GrangeasseSylvie Nessler
Apr 27, 2010·Nature Chemical Biology·Robert WoodwardPeng George Wang
May 19, 2010·Nature Chemical Biology·Chris Whitfield

❮ Previous
Next ❯

Citations

Mar 23, 2012·The Journal of Biological Chemistry·Sergei KalynychMiroslaw Cygler
Aug 15, 2012·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Christophe GrangeasseIvan Mijakovic
Dec 4, 2013·Antioxidants & Redox Signaling·Alistair J Standish, Renato Morona
Mar 2, 2016·FEMS Microbiology Reviews·Ivan MijakovicKürşad Turgay
Sep 13, 2015·Protein Expression and Purification·Chelsy Chesterman, Zongchao Jia
Jan 27, 2011·Biochimica Et Biophysica Acta·Ahasanul KobirIvan Mijakovic
May 1, 2016·Trends in Microbiology·Christophe Grangeasse
Aug 9, 2016·Molecular Oral Microbiology·C LiuR J Lamont
Dec 6, 2016·Acta Crystallographica. Section F, Structural Biology Communications·Marco Salomone-StagniStefano Benini
Jun 23, 2020·Annual Review of Biochemistry·Christopher A CaffaletteJochen Zimmer
Apr 2, 2013·The Journal of Biological Chemistry·Deniz B TemelRanajeet Ghose
Feb 21, 2014·The Journal of Biological Chemistry·Joseph D ChaoYossef Av-Gay
Jul 19, 2020·Annual Review of Microbiology·Chris WhitfieldCaitlin Sande
Mar 5, 2019·Biological & Pharmaceutical Bulletin·Hidesuke FukazawaYoshitsugu Miyazaki
Sep 7, 2018·Experimental & Molecular Medicine·Prachetash GhoshDong-Kwon Rhee
Apr 28, 2020·FEBS Letters·Julie Bonne KøhlerIvan Mijakovic
Nov 1, 2012·Emerging Microbes & Infections·Alain J Cozzone
Mar 7, 2019·Frontiers in Microbiology·Hyeongsoon KimSangryeol Ryu
Dec 19, 2020·FEMS Microbiology Reviews·Tong SuLok-To Sham
Jul 18, 2021·Nature Communications·Yun YangJames H Naismith
Oct 19, 2021·Frontiers in Molecular Biosciences·Fatlum Hajredini, Ranajeet Ghose
Nov 16, 2021·PloS One·Matthew R LarsonMark A Saper

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Respiration

This feed focuses on cellular respiration in bacteria, known as bacterial respiration. Discover the latest research here.