Identification of Suitable Endogenous Normalizers for qRT-PCR Analysis of Plasma microRNA Expression in Essential Hypertension

Molecular Biotechnology
Mohamed Hassan M SolaymanJulie A Johnson

Abstract

Circulating microRNAs (miRNAs) are promising biomarkers for many diseases. Quantitative reverse transcription polymerase chain reaction (qRT-PCR) is a gold standard for miRNA expression profiling that requires proper data normalization. Since there is no universal normalizer, it is recommended to evaluate normalizers under every experimental condition. This study describes the identification of suitable endogenous normalizer(s) (ENs) for plasma miRNA expression in essential hypertension. Expression levels of 5 candidate ENs and 2 plasma quality markers were determined by qRT-PCR in plasma samples from 18 hypertensive patients and 10 healthy controls. NormFinder, GeNorm, and DataAssist software programs were used to select the best EN(s). Expression levels of the 5 candidate ENs were also analyzed in urine samples from hypertensive patients and compared to the plasma samples of the hypertensive patients. Among the analyzed candidates, hsa-miR-92a-3p was identified as the best EN, and hsa-miR-21-5p and hsa-miR-16-5p as the next best. Moreover, hsa-miR-92a-3p showed the most consistent expression between plasma and urine. In conclusion, this study showed that hsa-miR-92a-3p, hsa-miR-21-5p, and hsa-miR-16-5p may be used as normaliz...Continue Reading

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Citations

Oct 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·G OcchipintiF Piva
Dec 10, 2016·Molecular Human Reproduction·Celia Corral-VazquezEster Anton
Jan 24, 2018·Metabolic Syndrome and Related Disorders·Elizabeth MaW Timothy Garvey
Mar 14, 2019·PloS One·Fernando Santamaria-MartosManuel Sánchez-de-la-Torre
Jul 6, 2018·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Xiao LiZuoguang Wang
May 16, 2019·Cellular and Molecular Life Sciences : CMLS·Soheil MadadiMeysam Soleimani
Jun 10, 2017·Current Hypertension Reports·Durairaj SekarAnupam J Das
Mar 20, 2019·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Hikaru NakashimaHideki Nakayama
Oct 20, 2019·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Vijayashree Priyadharsini Jayaseelan, Paramasivam Arumugam
Jul 3, 2021·Life·María Muñoz-San MartínLluís Ramió-Torrentà
Sep 12, 2021·European Journal of Clinical Nutrition·P KochharA Mukhopadhyay
Oct 7, 2021·European Journal of Clinical Nutrition·P KochharA Mukhopadhyay

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