Identification of sulfonylureas in serum by electrospray mass spectrometry

Analytical Biochemistry
F MagniM Galli Kienle

Abstract

Identification of sulfonylureas in serum is important in the diagnosis of hypoglycemic crisis of unknown origin. Methods based on HPLC with UV or fluorescence detection may give false positive results. Mass spectrometry may successfully avoid this problem. The described method allows the simultaneous identification and quantification of tolbutamide, chlorpropamide, glibenclamide, and glipizide in human serum using one of the tested sulfonylureas as the internal standard. Serum purification is carried out by solid-phase extraction with ENVI-C18 cartridges and samples are analyzed by liquid chromatography-electrospray mass spectrometry. For all drugs, the limit of detection and the limit of quantification are about 2 and 10 ng/ml, respectively.

Citations

Jul 22, 2005·The New England Journal of Medicine·Geoffrey J ServiceR V Lloyd
Dec 24, 2003·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Kenichi KumasakaShuji Satoh
Mar 23, 2007·Analytical and Bioanalytical Chemistry·Olaf H Drummer
Jan 23, 2016·Clinica Chimica Acta; International Journal of Clinical Chemistry·He S YangKara L Lynch
Dec 17, 2009·La Revue de médecine interne·C Bossu EstourL Fardet
Dec 9, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Isam Ismail SalemJaafar I Al Tamimi
Nov 25, 2017·Bioanalysis·Mariana Millan FachiRoberto Pontarolo
Dec 8, 2000·Journal of Mass Spectrometry : JMS
May 20, 2005·Endocrine Practice : Official Journal of the American College of Endocrinology and the American Association of Clinical Endocrinologists·Ananda BasuGeorge Eisenbarth

Related Concepts

Calibration
Insogen
Liquid Chromatography
Neogluconin
Glupitel
Mass Spectrometry
Sulfonylurea Compounds
Tolbutamid R.A.N.
Black Light, Ultraviolet

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