Identification of Tam41 maintaining integrity of the TIM23 protein translocator complex in mitochondria

The Journal of Cell Biology
Yasushi TamuraToshiya Endo

Abstract

Newly synthesized mitochondrial proteins are imported into mitochondria with the aid of protein translocator complexes in the outer and inner mitochondrial membranes. We report the identification of yeast Tam41, a new member of mitochondrial protein translocator systems. Tam41 is a peripheral inner mitochondrial membrane protein facing the matrix. Disruption of the TAM41 gene led to temperature-sensitive growth of yeast cells and resulted in defects in protein import via the TIM23 translocator complex at elevated temperature both in vivo and in vitro. Although Tam41 is not a constituent of the TIM23 complex, depletion of Tam41 led to a decreased molecular size of the TIM23 complex and partial aggregation of Pam18 and -16. Import of Pam16 into mitochondria without Tam41 was retarded, and the imported Pam16 formed aggregates in vitro. These results suggest that Tam41 facilitates mitochondrial protein import by maintaining the functional integrity of the TIM23 protein translocator complex from the matrix side of the inner membrane.

References

May 31, 2001·Methods in Cell Biology·H Schägger
Aug 23, 2002·Biochimica Et Biophysica Acta·Robert E Jensen, Cory D Dunn
Feb 13, 2003·Journal of Molecular Biology·Peter RehlingChristof Meisinger
Dec 24, 2003·Molecular Cell·Tony R HazbunTrisha N Davis
Feb 20, 2004·The Journal of Biological Chemistry·Nils WiedemannNikolaus Pfanner
Jun 15, 2004·Cancer Cell·Mary-Ann Bjornsti, Peter J Houghton
Jul 3, 2004·Nature Reviews. Molecular Cell Biology·Peter RehlingNikolaus Pfanner
Aug 25, 2004·The Journal of Cell Biology·Daigo IshikawaToshiya Endo
Sep 15, 2004·The Journal of Biological Chemistry·Mari NaoéToshiya Endo
Oct 12, 2004·Annual Review of Cell and Developmental Biology·Carla M Koehler
Oct 26, 2005·Biochemical Society Transactions·D Mokranjac, W Neupert

❮ Previous
Next ❯

Citations

Sep 11, 2007·Nature Cell Biology·Martin van der LaanPeter Rehling
Aug 24, 2010·Nature Reviews. Molecular Cell Biology·Oliver SchmidtChris Meisinger
Sep 18, 2012·The Journal of Biological Chemistry·Susanne E HorvathGünther Daum
Feb 6, 2008·The Journal of Biological Chemistry·Mahuya BoseHimangshu S Bose
Aug 31, 2012·Molecular Biology of the Cell·Takuya ShiotaToshiya Endo
Jan 8, 2010·Human Molecular Genetics·Devanjan SinhaPatrick D'Silva
Nov 4, 2009·Molecular and Cellular Biology·Agnieszka ChacinskaPeter Rehling
Feb 1, 2007·Annual Review of Biochemistry·Walter Neupert, Johannes M Herrmann
May 21, 2009·Biological Chemistry·Toshiya Endo, Koji Yamano
Oct 23, 2013·Chemistry and Physics of Lipids·Edgard M MejiaGrant M Hatch
Jan 22, 2014·Chemistry and Physics of Lipids·Vaishnavi Raja, Miriam L Greenberg
Nov 5, 2013·Chemistry and Physics of Lipids·Matthew G BaileSteven M Claypool
Apr 30, 2013·The Journal of Biological Chemistry·Thomas BeckerNikolaus Pfanner
Sep 26, 2013·Molecular and Cellular Biology·Gautam PareekPatrick D'Silva
Jan 12, 2011·The Journal of Cell Biology·Christof OsmanThomas Langer
Feb 25, 2009·The Journal of Cell Biology·Vishal M Gohil, Miriam L Greenberg
Dec 31, 2008·The Journal of Cell Biology·Stephan KutikNils Wiedemann
Jun 10, 2009·The Journal of Cell Biology·Yasushi TamuraHiromi Sesaki
Jul 19, 2011·Biochemical and Biophysical Research Communications·Yuuta UkaiYoshiharu Inoue
Mar 8, 2016·Biochimica Et Biophysica Acta·Neha ChauhanPeter Bütikofer
Jan 16, 2010·Biochemical and Biophysical Research Communications·Yoshihiro HaradaToshiya Endo
May 9, 2009·Current Opinion in Cell Biology·Thomas BeckerMartin van der Laan
Sep 6, 2011·Biochimica Et Biophysica Acta·Kai Stefan Dimmer, Doron Rapaport
Mar 1, 2014·International Journal of Cell Biology·Laila Cigana Schenkel, Marica Bakovic
Jan 27, 2010·Biochimica Et Biophysica Acta·Martin van der LaanPeter Rehling
Feb 2, 2010·Biochimica Et Biophysica Acta·Dejana Mokranjac, Walter Neupert
Jul 2, 2008·Biochimica Et Biophysica Acta·Karina WagnerPeter Rehling
Aug 2, 2008·Biochimica Et Biophysica Acta·Dejana Mokranjac, Walter Neupert
Apr 29, 2014·Progress in Lipid Research·Mindong RenMichael Schlame
Aug 11, 2010·Biochimica Et Biophysica Acta·Natalia GebertDiana Stojanovski
Feb 19, 2015·Journal of Bioenergetics and Biomembranes·Lena BöttingerThomas Becker
Jan 17, 2015·Trends in Endocrinology and Metabolism : TEM·Jared Rutter, Adam L Hughes
Jan 30, 2015·Journal of Bioenergetics and Biomembranes·Edgard M Mejia, Grant M Hatch
Aug 2, 2014·Journal of Inherited Metabolic Disease·Johannes A Mayr
Oct 28, 2016·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Kelly KeenanKristin M O'Brien
Feb 6, 2017·Cardiovascular Research·Jan Dudek, Christoph Maack
Oct 25, 2014·Nature Communications·Hsin-Hui ShenTrevor Lithgow
Dec 19, 2017·FEBS Letters·Ayaka MatsumuraToshihiko Oka

❮ Previous
Next ❯

Methods Mentioned

BETA
density gradient centrifugation
immunoprecipitation
coimmunoprecipitation
PCR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.