Identification of target genes for adenohypophysis-prefer miR-7 and miR-375 in cattle

Genetics and Molecular Research : GMR
Bao YuanJ B Zhang

Abstract

In this study, expression levels of miRNAs (miRNAs), miR-375 and miR-7, were detected in different tissues of cattle to determine whether adenohypophysis-prefer or exclusively expressed miRNAs, and target genes could be predicted by TargetScan, RNA22, and other software. Target genes related to pituitary function or reproductive traits were identified using a dual-luciferase assay. miR-375 and miR-7 were expressed differently in various tissues. miR-375 and miR-7 showed higher expression in the adenohypophysis, and there was a significant difference compared with expression in other tissues (P < 0.01). The binding sites for miR-7 were the mRNAs of bone morphogenetic protein receptor type II (BMPR2), prostaglandin F2 receptor negative regulator, gonadotropin-releasing hormone receptor, follicle-stimulating hormoneβ, somatostatin receptor 1, and interleukin-1β by bioinformatic analysis; similarly, the mRNAs of BMPR2 and leptin contained binding sites for miR-375, suggesting that these genes are affected by miR-7 or miR-375. Dual-luciferase reporter assays showed that miR-7 regulated prostaglandin F2 receptor negative regulator expression, while miR-375 regulated BMPR2 expression. The mutated plasmid and miRNA mimics were used to ...Continue Reading

Citations

Nov 14, 2019·BMC Genomics·Jennifer N KiserHolly L Neibergs
Oct 12, 2018·Journal of Leukocyte Biology·Fadoua BelaibaAmel Benammar Elgaaïed
Apr 4, 2021·Biomedicines·Petra KoraćMaja Matulić

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