Identification of testis-specific male contraceptive targets: insights from transcriptional profiling of the cycle of the rat seminiferous epithelium and purified testicular cells

Annals of the New York Academy of Sciences
Daniel S JohnstonWilliam W Wright

Abstract

In an effort to identify novel targets for the development of nonhormonal male contraceptives, genome-wide transcriptional profiling of the rat testis was performed. Specifically, enzymatically purified spermatogonia plus early spermatocyctes, pachytene spermatocytes, round spermatids, and Sertoli cells was analyzed along with microdissected rat seminiferous tubules at stages I, II-III, IV-V, VI, VIIa,b, VIIc,d, VIII, IX- XI, XII, XIII-XIV of the cycle of the seminiferous epithelium using RAE 230_2.0 microarrays. The combined analysis of these studies identified 16,971 expressed probe sets on the array. How these expression data, combined with additional bioinformatic data analysis and quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) analysis, led to the identification of 58 genes that have 1000-fold higher expression transcriptionally in the testis when compared to over 20 other nonreproductive tissues is described. The products of these genes may play important roles in testicular and/or sperm function, and further investigation on their utility as nonhormonal contraceptive targets is warranted. Moreover, these microarray data have been used to expedite the identification of a mutation in RIKEN cDNA 2410...Continue Reading

Citations

Jun 25, 2010·Reproductive Biology and Endocrinology : RB&E·Nafisa H BalasinorMartin Dym
Nov 10, 2011·PloS One·Damian B YapSamuel Aparicio
Jan 18, 2011·Journal of Reproductive Immunology·E Dimitriadis, E Menkhorst
Jun 21, 2020·Biology of Reproduction·Daniel S Johnston, Erwin Goldberg
Feb 21, 2018·Biology of Reproduction·Michael D Griswold

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