Identification of the 2-tridecanone cis-acting element in the promoter of cytochrome P450 CYP6B7 in Helicoverpa armigera

Insect Science
Li XuLihong Qiu

Abstract

The expression level of cytochrome P450 genes in insects can be induced by plant allelochemicals, which is important for insects to adapt to host plants. Cytochrome P450 CYP6B7 has been reported to be involved in pyrethroid insecticide resistance in Helicoverpa armigera, and its transcription level was induced by some inducers. Currently, the regulatory mechanism of the induced expression of CYP6B7 remains unknown, although it is very important for understanding the detoxification mechanism to allelochemicals in host plants. The objective of the present study was to investigate the cis-acting element in the promoter of CYP6B7 mediating the inducible up-regulation of CYP6B7 in H. armigera by 2-tridecanone. The promoter region of CYP6B7 was cloned by genome walking technique and analyzed by transient transfection assay. Progressive 5' deletion of the promoter region of CYP6B7 revealed that the relative luciferase activity of construct -320/+232 could be significantly induced by 2-tridecanone. Further stepwise deletion between -320 and -238 bp found that construct -292/+232 could also be significantly induced by 2-tridecanone, but the adjacent construct -256/+232 could not, suggesting the essential role of the sequence between -29...Continue Reading

References

Jan 1, 1993·Insect Biochemistry and Molecular Biology·C AntoniewskiJ A Lepesant
Jun 14, 2000·Insect Biochemistry and Molecular Biology·J L StevensR Feyereisen
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Jun 7, 2002·Journal of Chemical Ecology·May R Berenbaum
Feb 8, 2003·Biochimica Et Biophysica Acta·Rebecca A PetersenMary A Schuler
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
Sep 22, 2010·Insect Biochemistry and Molecular Biology·Chunni ZhangXianchun Li

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Citations

Jan 2, 2021·Current Opinion in Insect Science·Xianchun LiXuewei Chen
Dec 30, 2020·Current Opinion in Insect Science·Marilou VandenholeThomas Van Leeuwen

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