PMID: 2119500Sep 1, 1990Paper

Identification of the albino mutation of mouse tyrosinase by analysis of an in vitro revertant

Proceedings of the National Academy of Sciences of the United States of America
I J Jackson, D C Bennett

Abstract

From within an albino melanocyte line grown in vitro we identified and cloned cells that apparently had reverted to wild type. We sequenced a part of the tyrosinase gene, encompassing a candidate mutation, from wild-type, albino, and revertant cell DNAs. The revertant cells contain, on one chromosome, a perfect base reversion to the wild-type sequence of this candidate mutation, proving that this is the sole defect in the tyrosinase gene of albino mutant mice. The revertant cells readily regain the albino phenotype after freezing and thawing. Taking advantage of a Dde I restriction site created by the albino mutation, we demonstrated that the regained phenotype is due to allele loss involving the wild-type chromosome. The Dde I site also allowed us to show that all inbred albino mice carry the same mutation and so must be derived from the same progenitor.

References

Mar 15, 1989·Biochemical and Biophysical Research Communications·M TeraoB Mintz
Mar 1, 1989·Pigment Cell Research·V J Hearing, M Jiménez
Nov 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B S KwonR Halaban
Oct 22, 1985·Biochemistry·M HuberK Lerch
Mar 15, 1987·International Journal of Cancer. Journal International Du Cancer·D C BennettI R Hart
Jun 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·I J Jackson
Jun 30, 1988·Biochemical and Biophysical Research Communications·B S KwonD Kestler
Sep 29, 1988·Nature·B Ponder
Jul 1, 1987·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·A TamuraA B Lerner
Apr 1, 1973·Journal of Ultrastructure Research·V J HearingM A Lutzner
Nov 1, 1967·The Journal of Cell Biology·P F Parakkal

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Citations

Sep 1, 1991·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·I J Jackson
Nov 1, 1996·Transgenic Research·B AignerG Brem
Dec 1, 1992·Mutation Research·J Favor, C Morawetz
May 25, 2002·Pigment Cell Research·José C García-Borrón, Francisco Solano
Jul 9, 2002·Pigment Cell Research·Rong HanLorin Weiner
Jul 9, 2002·Pigment Cell Research·Patricia Giraldo, Lluís Montoliu
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B Mintz, M Bradl
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·F BeermannG Schütz
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·A Schmidt, F Beermann
May 15, 2002·Cloning and Stem Cells·Lluís Montoliu
Apr 1, 1994·Pigment Cell Research·I J JacksonK Steel
Apr 14, 2005·BMC Cell Biology·Alfonso LavadoLluís Montoliu
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·R HalabanD N Hebert
Jun 1, 1995·Current Opinion in Genetics & Development·B T Lamb, J D Gearhart
Aug 23, 2002·The Journal of Investigative Dermatology·Ruth HalabanDaniel N Hebert
Sep 1, 1992·Archives of Biochemistry and Biophysics·P ValverdeJ A Lozano
Apr 12, 2008·BMC Genetics·Michael G AndersonSimon W M John
Jul 10, 2010·PLoS Genetics·Colleen M TrantowMichael G Anderson
Nov 14, 2016·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Xiajun ChenJiale Li
May 10, 2017·Human Molecular Genetics·Kacie J Meyer, Michael G Anderson
Nov 19, 2003·The European Journal of Neuroscience·Estela GiménezLluís Montoliu
Mar 1, 1997·Developmental Dynamics : an Official Publication of the American Association of Anatomists·S SatoH Yamamoto
Aug 1, 1993·Pigment Cell Research·P A Riley
Jul 16, 2003·Pigment Cell Research·Dorothy C Bennett, M Lynn Lamoreux
Jan 1, 1992·Pigment Cell Research·R Halaban, G Moellmann
Mar 8, 2003·Pigment Cell Research·Elodie BietLionel Larue
Jun 21, 2001·Genesis : the Journal of Genetics and Development·K J DunnW J Pavan
Oct 27, 2020·Genetics in Medicine : Official Journal of the American College of Medical Genetics·Perrine PennamenBenoit Arveiler

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