Identification of the amino acid region involved in the intercellular interaction between the β1 subunits of Na+/K+ -ATPase.

Journal of Cell Science
Elmira TokhtaevaOlga Vagin

Abstract

Epithelial junctions depend on intercellular interactions between β(1) subunits of the Na(+)/K(+)-ATPase molecules of neighboring cells. The interaction between dog and rat subunits is less effective than the interaction between two dog β(1) subunits, indicating the importance of species-specific regions for β(1)-β(1) binding. To identify these regions, the species-specific amino acid residues were mapped on a high-resolution structure of the Na(+)/K(+)-ATPase β(1) subunit to select those exposed towards the β(1) subunit of the neighboring cell. These exposed residues were mutated in both dog and rat YFP-linked β(1) subunits (YFP-β(1)) and also in the secreted extracellular domain of the dog β(1) subunit. Five rat-like mutations in the amino acid region spanning residues 198-207 of the dog YFP-β(1) expressed in Madin-Darby canine kidney (MDCK) cells decreased co-precipitation of the endogenous dog β(1) subunit with YFP-β(1) to the level observed between dog β(1) and rat YFP-β(1). In parallel, these mutations impaired the recognition of YFP-β(1) by the dog-specific antibody that inhibits cell adhesion between MDCK cells. Accordingly, dog-like mutations in rat YFP-β(1) increased both the (YFP-β(1))-β(1) interaction in MDCK cells ...Continue Reading

References

May 20, 2003·American Journal of Physiology. Lung Cellular and Molecular Physiology·Hong HaoDavid H Ingbar
Jun 30, 2004·Journal of Molecular Biology·Jannick Dyrløv BendtsenSøren Brunak
Nov 25, 2004·Seminars in Cell & Developmental Biology·Kenji IrieYoshimi Takai
Apr 12, 2005·Advanced Drug Delivery Reviews·Jun Miyoshi, Yoshimi Takai
Jul 19, 2005·Nature Reviews. Molecular Cell Biology·Barry M Gumbiner
Oct 21, 2006·The Journal of Biological Chemistry·Olga VaginGeorge Sachs
Jul 11, 2007·The Journal of Biological Chemistry·Krishnakumar KizhatilVann Bennett
Sep 8, 2007·American Journal of Nephrology·Jun Miyoshi, Yoshimi Takai
Sep 15, 2007·Biochimica Et Biophysica Acta·Andrea Hartsock, W James Nelson
Oct 19, 2007·Current Opinion in Cell Biology·Toshiaki SakisakaYoshimi Takai
May 22, 2009·Nature·Takehiro ShinodaChikashi Toyoshima
Jan 12, 2010·Trends in Cell Biology·Emily SteedKarl Matter
Jan 13, 2010·Cold Spring Harbor Perspectives in Biology·Lawrence Shapiro, William I Weis
May 7, 2010·Molecular Biology of the Cell·Teresita Padilla-BenavidesLiora Shoshani
Sep 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Yinghao WuAvinoam Ben-Shaul
Mar 23, 2011·The Journal of Cell Biology·Buzz Baum, Marios Georgiou
Jun 7, 2011·The Journal of Biological Chemistry·Elmira TokhtaevaOlga Vagin

❮ Previous
Next ❯

Citations

Jan 27, 2012·American Journal of Physiology. Cell Physiology·Olga VaginGeorge Sachs
Feb 9, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Li-Yen R Hu, Aikaterini Kontrogianni-Konstantopoulos
Jun 15, 2012·Cellular and Molecular Life Sciences : CMLS·Linda ReinhardPoul Nissen
Dec 9, 2014·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Paula ArmestoManuel Manchado
Aug 1, 2018·International Journal of Molecular Sciences·Thomas LiebmannHjalmar Brismar
May 2, 2019·The Journal of Membrane Biology·O Verdejo-TorresR G Contreras
Mar 17, 2020·American Journal of Physiology. Gastrointestinal and Liver Physiology·Elizabeth A MarcusOlga Vagin
Jan 13, 2015·The Journal of Biological Chemistry·Elmira TokhtaevaOlga Vagin
Jun 12, 2021·The Journal of Membrane Biology·Vitalii KryvenkoIstván Vadász

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.