Identification of the Anabaena sp. strain PCC7120 cyanophycin synthetase as suitable enzyme for production of cyanophycin in gram-negative bacteria like Pseudomonas putida and Ralstonia eutropha

Biomacromolecules
Ingo VossA Steinbüchel

Abstract

The cyanophycin synthetase gene cphA1 encoding the major cyanophycin synthetase (CphA) of Anabaena sp. strain PCC7120 was expressed in Escherichia coli conferring so far the highest specific CphA activity to E. coli (6.7 nmol arginine per min and mg protein). CphA1 and cphA genes of Synechocystis sp. strains PCC6803 and PCC6308 and Synechococcus strain MA19 were also expressed in wild types and polyhydroxyalkanoate-negative (PHA) mutants of Pseudomonas putida and Ralstonia eutropha. Recombinant strains of these bacteria expressing cphA1 accumulated generally more cyanophycin (23.0 and 20.0% of cellular dry matter, CDM, respectively) than recombinants expressing any other cphA (6.8, 9.0, or 15.8% of CDM for P. putida strains and 7.3, 12.6, or 14.1% of CDM for R. eutropha). Furthermore, PHA-negative mutants of P. putida (9.7, 10.0, 17.5, or 24.0% of CDM) and R. eutropha (8.9, 13.8, 16.0, or 22.0% of CDM) accumulated generally more cyanophycin than the corresponding PHA-positive parent strains (6.8, 9.0, 15.8, and 23.0% of CDM for P. putida strains and 7.3, 12.6, 14.1, or 20.0% of CDM for R. eutropha strains). Recombinant strains of Gram-positive bacteria (Bacillus megaterium, Corynebacterium glutamicum) were not suitable for cyan...Continue Reading

References

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Jun 29, 2002·Applied and Environmental Microbiology·Kay M FreyAlexander Steinbüchel

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Citations

Mar 28, 2007·Applied Microbiology and Biotechnology·Elinor ScottJohan Sanders
Aug 6, 2008·Applied Microbiology and Biotechnology·Toshinobu Arai, Kuniki Kino
Oct 13, 2010·Applied Microbiology and Biotechnology·Yasser Abd-El-KaremAlexander Steinbüchel
Oct 6, 2010·Applied Microbiology and Biotechnology·Jens KrollAlexander Steinbüchel
Apr 2, 2011·Applied Microbiology and Biotechnology·Lars WiefelAlexander Steinbüchel
Oct 6, 2011·Applied Microbiology and Biotechnology·Bas J MeussenLeo H de Graaff
Dec 12, 2012·Applied Microbiology and Biotechnology·Katja AdamesAlexander Steinbüchel
Apr 13, 2013·Applied Microbiology and Biotechnology·Jikun DuShining Zhou
Apr 15, 2008·Applied and Environmental Microbiology·Anna SteinleAlexander Steinbüchel
Sep 4, 2012·Applied and Environmental Microbiology·Steffen LütteBärbel Friedrich
Dec 21, 2010·Applied and Environmental Microbiology·Shanna SichwartAlexander Steinbüchel
Dec 8, 2005·Applied and Environmental Microbiology·Yasser ElbahloulAlexander Steinbüchel
Feb 8, 2006·Applied and Environmental Microbiology·Yasser Elbahloul, Alexander Steinbüchel
Feb 14, 2007·Macromolecular Bioscience·Johan SandersHans Mooibroek
Jun 24, 2015·Applied Microbiology and Biotechnology·Anita Loeschcke, Stephan Thies
Dec 14, 2017·Critical Reviews in Biotechnology·Matthias RabergAlexander Steinbüchel
Dec 4, 2014·Microbial Cell Factories·Jae Ho Shin, Sang Yup Lee

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