Identification of the binding site on S100B protein for the actin capping protein CapZ

Protein Science : a Publication of the Protein Society
P M KilbyG C Roberts

Abstract

The calcium-binding protein S100B binds to several potential target proteins, but there is no detailed information showing the location of the binding site for any target protein on S100B. We have made backbone assignments of the calcium-bound form of S100B and used chemical-shift changes in spectra of 15N-labeled protein to locate the site that binds a peptide corresponding to residues 265-276 from CapZ alpha, the actin capping protein. The largest chemical-shift changes are observed for resonances arising from residues around the C terminus of the C-terminal helix of S100B and residues Val-8 to Asp-12 of the N-terminal helix. These residues are close to but not identical to residues that have been identified by mutational analysis to be important in other S100 protein-protein interactions. They make up a patch across the S100B dimer interface and include some residues that are quite buried in the structure of calcium-free S100B. We believe we may have identified a binding site that could be common to many S100 protein-protein interactions.

References

Nov 10, 1992·Biochimica Et Biophysica Acta·R BianchiR Donato
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J BaudierJ J Lawrence
Jan 1, 1990·Journal of Biochemistry·T FujiiY Kondo
Jul 1, 1994·Protein Science : a Publication of the Protein Society·M HensmannI D Campbell
Jun 16, 1995·The Journal of Biological Chemistry·V V IvanenkovR V Dimlich
Jan 1, 1995·Brain Research Bulletin·D B ZimmerW Song
Jun 2, 1995·Journal of Molecular Biology·N J SkeltonW J Chazin
Jul 1, 1994·Journal of Biochemistry·T FujiiY Kondo
Sep 1, 1994·Brain Research. Molecular Brain Research·L H LinJ J Norden
Nov 15, 1993·Biochemical and Biophysical Research Communications·M WatanabeH Hidaka
Apr 1, 1993·Cell Calcium·D B Zimmer, J G Dubuisson
Apr 18, 1996·Nature·J HeierhorstB E Kemp
Apr 1, 1996·Trends in Biochemical Sciences·B W Schäfer, C W Heizmann

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Citations

Jan 25, 2006·Proceedings of the National Academy of Sciences of the United States of America·Mitsuhiko Ikura, James B Ames
Dec 21, 2002·Protein Science : a Publication of the Protein Society·Małgorzata PalczewskaJacek Kuźnicki
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Kathleen A ClarkCarol C Gregorio
Jul 31, 2010·Cardiovascular Psychiatry and Neurology·James N TsoporisThomas G Parker
Mar 30, 2011·PloS One·Eva ThulinSara Linse
Mar 6, 2010·Amino Acids·James N TsoporisThomas G Parker
Jan 9, 1999·The Journal of Biological Chemistry·K R BarberG S Shaw
Jul 27, 2000·The Journal of Biological Chemistry·J C DeloulmeJ Baudier
Mar 20, 2003·Microscopy Research and Technique·Matthias RothermundtVolker Arolt
Feb 18, 2004·The Journal of Biological Chemistry·Peter L Ferguson, Gary S Shaw
Dec 8, 2000·Biochimica Et Biophysica Acta·M GarbugliaR Donato
Aug 13, 2008·Biochemical and Biophysical Research Communications·Marleen Van TroysJoël Vandekerckhove

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