Identification of the Biosynthetic Gene Cluster for the Anti-infective Desotamides and Production of a New Analogue in a Heterologous Host

Journal of Natural Products
Qinglian LiJianhua Ju

Abstract

The desotamides (DSAs) are potent antibacterial cyclohexapeptides produced by Streptomyces scopuliridis SCSIO ZJ46. We have identified the 39-kb dsa biosynthetic gene cluster by whole-genome scanning. Composed of 17 open reading frames, the cluster codes for four nonribosomal peptide synthetases and associated resistance, transport, regulatory, and precursor biosynthesis proteins. Heterologous expression of the dsa gene cluster in S. coelicolor M1152 afforded desotamides A and B and the new desotamide G. Cluster identification and its demonstrated amenability to heterologous expression provide the foundation for future mechanistic studies as well as the generation of new and potentially clinically significant DSA analogues.

References

Sep 27, 2002·Chembiochem : a European Journal of Chemical Biology·Henning D MootzMohamed A Marahiel
Feb 4, 2003·Proceedings of the National Academy of Sciences of the United States of America·Bertolt GustKeith F Chater
Nov 1, 2003·FEMS Microbiology Letters·Oleg KurnasovAndrei Osterman
Feb 24, 2011·Microbial Biotechnology·Juan Pablo Gomez-Escribano, Mervyn J Bibb
Sep 18, 2014·Organic Letters·Zeinab G KhalilRobert J Capon

❮ Previous
Next ❯

Citations

Feb 11, 2016·Journal of Natural Products·Soumini VijayasarathyRobert J Capon
Dec 17, 2015·Journal of the American Chemical Society·Qinglian LiJianhua Ju
Jun 4, 2016·Archiv der Pharmazie·Paulina BekieschAlexander K Apel
May 8, 2019·Chemical & Pharmaceutical Bulletin·Kenichi MatsudaToshiyuki Wakimoto
Nov 28, 2019·Applied Microbiology and Biotechnology·Zhijie YangJunying Ma
Jan 15, 2019·Organic & Biomolecular Chemistry·Kenichi MatsudaToshiyuki Wakimoto
Mar 16, 2017·Natural Product Reports·John W BluntMichèle R Prinsep
Jul 30, 2019·International Journal of Microbiology·Periyasamy SivalingamKandasamy Prabakar
Jul 31, 2020·Antibiotics·Asif FazalRyan F Seipke
May 17, 2017·Frontiers in Microbiology·Manita KamjamKui Hong
Mar 14, 2021·Journal of Industrial Microbiology & Biotechnology·Kenichi MatsudaToshiyuki Wakimoto
Mar 30, 2019·ACS Chemical Biology·Divya ThankachanRyan F Seipke
Jun 12, 2019·Journal of Natural Products·Cynthia M MudalunguRoderich D Süssmuth
Dec 24, 2019·Chemical Reviews·Jason B Hedges, Katherine S Ryan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.