Identification of the Brucella melitensis vaccine strain Rev.1 in animals and humans in Israel by PCR analysis of the PstI site polymorphism of its omp2 gene.

Journal of Clinical Microbiology
Svetlana BardensteinMenachem Banai

Abstract

Adverse effects of strain persistence and secretion in milk have been encountered with the Brucella melitensis vaccine strain Rev.1. Field isolates obtained from vaccinated animals and from a human resembled the vaccine strain Rev.1 by conventional bacteriological tests. The lack of a specific molecular marker that could specifically characterize the commercial vaccine strain prevented confirmation of the homology of the Rev.1-like field isolates to the vaccine strain. The composition of the omp2 locus from two gene copies with differences in their PstI restriction endonuclease sites was used to establish an epidemiologic fingerprint for the omp2 gene in the Rev.1 vaccine strain. Primers designed to amplify DNA sequences that overlap the PstI site revealed a single 282-bp DNA band common to all Brucella spp. Agarose gel electrophoresis of the PstI digests of the PCR products from strains 16M and the vaccine strain Rev.1 revealed a distinctive profile that included three bands: one band for the intact 282-bp fragment amplified from omp2a and two bands resulting from the digestion of the amplified omp2b gene fragment, 238- and 44-bp DNA fragments, respectively. Amplified fragments of 37 Rev.1-like isolates, including 2 human isol...Continue Reading

References

Jan 1, 1992·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·A FeketeS M Halling
Oct 1, 1991·Biologicals : Journal of the International Association of Biological Standardization·N Bosseray
Jan 1, 1987·Methods in Enzymology·K B Mullis, F A Faloona
Jan 1, 1996·International Journal of Systematic Bacteriology·T A FichtS W Bearden
Dec 1, 1995·Journal of Clinical Microbiology·D S Leal-KlevezasJ P Martínez-Soriano
Nov 24, 1999·Journal of Clinical Microbiology·P MorataJ D Colmenero
Dec 29, 2000·The Veterinary Record·A GuarinoP Gallo

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Citations

Oct 25, 2011·Veterinary Research Communications·Georgios T NoutsiosDimitrios A Kyriakidis
Nov 5, 2002·Veterinary Microbiology·Gerhardt G SchurigMichael J Corbel
Jul 29, 2003·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Ruth L Berkelman
Jan 30, 2009·The Pediatric Infectious Disease Journal·Bat-Chen FriedmanMoshe Ephros
Jan 8, 2009·Journal of Immune Based Therapies and Vaccines·Jerome S HarmsGary A Splitter
Sep 18, 2008·Journal of the American Veterinary Medical Association·M Kathleen Glynn, Tracey V Lynn
Nov 6, 2010·Veterinary Medicine International·Assadullah SamadiS Q Lafi
Apr 5, 2012·Veterinary Immunology and Immunopathology·A Q AbbadyS Muyldermans
Aug 2, 2014·Annals of Clinical Microbiology and Antimicrobials·Ying WangHui Yu
Feb 14, 2006·BMC Microbiology·Philippe Le FlècheGilles Vergnaud
Nov 22, 2008·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Cláudia AlmendraGordon Luikart
Dec 24, 2005·Expert Review of Vaccines·Steven C Olsen, W S Stoffregen
Aug 24, 2007·Clinical and Vaccine Immunology : CVI·M Victoria DelpinoPablo C Baldi
Sep 8, 2021·Frontiers in Microbiology·Kumaragurubaran KarthikGopal Dhinakar Raj
May 17, 2006·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·N E LuceroI Jacques

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