Identification of the CD8 DE loop as a surface functional epitope. Implications for major histocompatibility complex class I binding and CD8 inhibitor design.

The Journal of Biological Chemistry
S LiZ Huang

Abstract

We used an approach of protein surface epitope mapping by synthetic peptides to analyze the surface structure-function relationship of the CD8 protein. Small synthetic peptide mimics of the CD8 DE loop were shown to effectively block CD8 binding to major histocompatibility complex (MHC) class I molecules and possess significant inhibitory activity on in vitro CD8(+) T cell function. These results suggested that the DE loop region of the CD8 protein is an important functional epitope mediating CD8-MHC class I interaction and the activation of CD8(+) T cells, a finding that is consistent with the recently reported crystal structure of the CD8-MHC class I complex. The structural basis for the biological activity of the DE loop peptide was further analyzed in a series of analogs containing alanine substitutions. This study provides support for the concept of bioactive peptide design based on protein surface epitopes and suggests that such an approach may be applicable to other protein-protein complexes, particularly those of immunoglobulin superfamily molecules.

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Citations

Jun 27, 2002·Trends in Pharmacological Sciences·Niki Zacharias, Dennis A Dougherty
Jan 1, 2009·Advances and Applications in Bioinformatics and Chemistry : AABC·Solène GrosdidierJuan Fernández-Recio
Jun 1, 2009·Expert Opinion on Drug Discovery·Solène Grosdidier, Juan Fernández-Recio
Sep 25, 2004·Biology of Blood and Marrow Transplantation : Journal of the American Society for Blood and Marrow Transplantation·Swati ChoksiRobert Korngold
Oct 8, 1999·Biochemical and Biophysical Research Communications·Z LuoZ Huang

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