Identification of the components of bitespiramycin by liquid chromatography-mass spectrometry

Journal of Pharmaceutical and Biomedical Analysis
Ming-juan WangErwin Adams

Abstract

Reversed-phase liquid chromatography coupled with electrospray ionization tandem mass spectrometry (LC/ESI-MS/MS) was used to characterize the components of bitespiramycin, a group of 4"-acylated spiramycins produced by bioengineered strains. In total 38 components were characterized in commercial samples, including 12 impurities that had never been reported before and 12 other that were partially characterized. The structures of these unknown compounds were deduced by comparison of their fragmentation patterns with those of known major components. Their ultraviolet spectra were used to confirm the presence of an α-, β-, γ-, δ-unsaturated butadiene in the macrocyclic lactone. Compared with the classical method, LC/ESI-MS/MS is particularly advantageous in terms of sensitivity and efficiency to characterize minor components at trace levels in multi-component antibiotics.

References

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Jan 31, 2007·Rapid Communications in Mass Spectrometry : RCM·Murali PendelaErwin Adams
Sep 17, 2010·Journal of Chromatography. a·Ming-juan WangErwin Adams
Dec 21, 2013·Archives of Microbiology·Yang DengXiaomei Bie

Citations

Mar 26, 2016·Chemistry & Biodiversity·Johannes Niebler, Andrea Buettner
Mar 26, 2016·Chemistry & Biodiversity·Johannes NieblerAndrea Buettner
Apr 23, 2016·Chemistry & Biodiversity·Anne-Marie NamAnge Bighelli
May 1, 2018·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Keila Fernanda RaimundoZilda Cristiani Gazim

Related Concepts

Bitespiramycin
Antibiotics
Liquid Chromatography
Drug Impurity
Rovamycine
Spectrometry, Mass, Electrospray Ionization
Tandem Mass Spectrometry
Chromatography, Reversed-Phase Liquid
Medical Devices
Spiramycin

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