Identification of the core element responsive to runt-related transcription factor 2 in the promoter of human type X collagen gene

Arthritis and Rheumatism
Akiro HigashikawaHiroshi Kawaguchi

Abstract

Type X collagen and runt-related transcription factor 2 (RUNX-2) are known to be important for chondrocyte hypertrophy during skeletal growth and repair and development of osteoarthritis (OA) in mice. Aiming at clinical application, this study was undertaken to investigate transcriptional regulation of human type X collagen by RUNX-2 in human cells. Localization of type X collagen and RUNX-2 was determined by immunohistochemistry, and their functional interaction was examined in cultured mouse chondrogenic ATDC-5 cells. Promoter activity of the human type X collagen gene (COL10A1) was examined in human HeLa, HuH7, and OUMS27 cells transfected with a luciferase gene containing a 4.5-kb promoter and fragments. Binding to RUNX-2 was examined by electrophoretic mobility shift assay and chromatin immunoprecipitation. RUNX-2 and type X collagen were co-localized in mouse limb cartilage and bone fracture callus. Gain and loss of function of RUNX-2 revealed that RUNX-2 is essential for type X collagen expression and terminal differentiation of chondrocytes. Human COL10A1 promoter activity was enhanced by RUNX-2 alone and more potently by RUNX-2 in combination with the coactivator core-binding factor beta in all 3 human cell lines exami...Continue Reading

References

Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Oct 1, 1994·Nature Genetics·R RosatiR R Behringer
Sep 1, 1993·Nature Genetics·M L WarmanC A Francomano
Nov 1, 1995·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M IwamotoM Pacifici
Mar 1, 1997·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·R J O'KeefeR N Rosier
Aug 1, 1997·Journal of Cellular Biochemistry·F BeierM J Lammi
May 22, 1998·Progress in Nucleic Acid Research and Molecular Biology·T F LinsenmayerT M Schmid
Aug 8, 1998·International Journal of Hematology·T Kitamura
Aug 26, 1998·Matrix Biology : Journal of the International Society for Matrix Biology·D Chan, O Jacenko
Mar 12, 1999·Mechanisms of Development·I S KimS Mundlos
Apr 23, 1999·Developmental Dynamics : an Official Publication of the American Association of Anatomists·M InadaT Komori
Aug 25, 1999·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·N BoosM Aebi
Mar 18, 2000·The Journal of Biological Chemistry·H EnomotoT Komori
Feb 22, 2002·Human Mutation·Florian OttoStefan Mundlos
May 16, 2003·Nature·Henry M Kronenberg
Aug 22, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D MagneJ Guicheux
Oct 7, 2004·Matrix Biology : Journal of the International Society for Matrix Biology·Sonja GebhardKlaus von der Mark
May 18, 2005·Osteoarthritis and Cartilage·S KamekuraH Kawaguchi
Aug 20, 2005·The Journal of Biological Chemistry·Fumitaka KugimiyaUng-il Chung
May 9, 2006·Nature Medicine·Takashi YamadaHiroshi Kawaguchi
Jun 24, 2006·Journal of Cellular Biochemistry·Toshihisa Komori

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Citations

Aug 4, 2009·Cell and Tissue Research·Toshihisa Komori
Jul 8, 2010·Journal of Bone and Mineral Metabolism·Hironori HojoUng-il Chung
Jul 16, 2013·Molecules and Cells·Yong-Il KimCheol-Hee Kim
Aug 8, 2012·Stem Cell Reviews and Reports·Andrea PreitschopfMario Mikula
Sep 6, 2011·The Journal of Biological Chemistry·Noboru TaniguchiMartin Lotz
Dec 2, 2015·European Journal of Oral Sciences·Naser A AminabadiSajjad Shirazi
Sep 3, 2011·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Feifei LiQiping Zheng
Nov 26, 2013·Mechanisms of Development·Rainer Schmid, Anja-Katrin Bosserhoff
Dec 20, 2011·Osteoarthritis and Cartilage·P M van der Kraan, W B van den Berg
Aug 16, 2016·Annals of the New York Academy of Sciences·Weiwei ZhaoDi Chen
Dec 19, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Yugo MoritaShuichi Matsuda
Jun 13, 2019·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sarah E CathelineJennifer H Jonason
May 31, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Min-Jung ParkMi-La Cho
Mar 25, 2020·Biological Reviews of the Cambridge Philosophical Society·Jian-Fei YanKai Jiao
Aug 5, 2020·Journal of Experimental & Clinical Cancer Research : CR·Ting WangYujun Hao
Jul 17, 2014·The Journal of Biological Chemistry·Katsuhiko AmanoBeate Lanske
Mar 30, 2018·Frontiers in Bioengineering and Biotechnology·Swarna RamanJ Mary Murphy
Apr 18, 2019·Calcified Tissue International·Masahiro HoritaToshifumi Ozaki
Aug 15, 2019·Laboratory Investigation; a Journal of Technical Methods and Pathology·Lyess AllasCatherine Baugé
Mar 21, 2020·Frontiers in Cell and Developmental Biology·Simon I DreherWiltrud Richter
May 28, 2021·Frontiers in Cell and Developmental Biology·Xiwei FanIndira Prasadam

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