Identification of the cutaneous basement membrane zone antigen and isolation of antibody in linear immunoglobulin A bullous dermatosis

The Journal of Clinical Investigation
J J ZoneL J Meyer

Abstract

Linear IgA bullous dermatosis (LABD) is a rare blistering skin disease characterized by basement membrane zone deposition of IgA. This study identifies a tissue antigen detected by patient serum and then isolates the autoantibody using epidermis and protein bands blotted on nitrocellulose as immunoabsorbents. Sera from 10 patients (9 with cutaneous disease and 1 with cicatrizing conjunctivitis) were evaluated. Indirect immunofluorescence revealed an IgA anti-basement membrane antibody in 6 of 10 sera with monkey esophagus substrate and 9 of 10 sera with human epidermal substrate. Immunoblotting was performed on epidermal and dermal extracts prepared from skin separated at the basement membrane zone with either sodium chloride or EDTA. Saline-separated skin expressed a 97-kD band in dermal extract alone that was recognized by 4 of 10 sera. EDTA-separated skin expressed the 97-kD band in both epidermal (4 of 10 sera) and dermal (6 of 10 sera) extract. Immunoabsorption of positive sera with epidermis purified an IgA antibody that reacted uniquely with the 97-kD band. In addition, IgA antibody bound to nitrocellulose was eluted from the 97-kD band and found to uniquely bind basement membrane zone. It is likely that the 97-kD protei...Continue Reading

References

Feb 1, 1979·Archives of Dermatology·T T ProvostR L Dobson
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Mar 1, 1988·The Journal of Clinical Investigation·D T WoodleyR A Briggaman
Jun 1, 1987·Journal of the American Academy of Dermatology·R P Hall
Sep 1, 1987·The British Journal of Dermatology·B BhogalP H McKee
Apr 19, 1984·The New England Journal of Medicine·D T WoodleyW R Gammon
Sep 1, 1982·The British Journal of Dermatology·J N LeonardL Fry

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Citations

Jan 1, 1996·Molecular Biology Reports·J Uitto, L Pulkkinen
Jan 1, 1995·Archives of Dermatological Research·C KowalewskiD Schmitt
Jan 1, 1993·Archives of Dermatological Research·A Mohimen, A R Ahmed
May 11, 2012·Seminars in Immunopathology·Eric V Marietta, Joseph A Murray
Aug 15, 1992·Lancet·L S Chan, K D Cooper
Jul 1, 1996·Clinics in Dermatology·M C Ray, L E Gately
Dec 1, 1993·Journal of Dermatological Science·M DmochowskiT Nishikawa
Dec 1, 1995·Current Opinion in Immunology·H C Nousari, G J Anhalt
Feb 10, 2000·The American Journal of Pathology·H SchumannL Bruckner-Tuderman
Jan 3, 2001·Clinics in Dermatology·K E Bowers
Oct 18, 2001·Clinics in Dermatology·A Ishiko, H Shimizu
Nov 14, 2001·Clinics in Dermatology·T Olivry, L S Chan
Jun 24, 1999·Journal of Dermatological Science·D Zillikens
Apr 24, 1999·The British Journal of Dermatology·A ArechaldeL Borradori
Jun 3, 1999·The British Journal of Dermatology·C A EganJ J Zone
Jun 3, 1999·The British Journal of Dermatology·E SchmidtD Zillikens
Mar 29, 2000·The British Journal of Dermatology·Y TakagiM Niimura
May 16, 2000·The British Journal of Dermatology·D LipskerE Grosshans
Jan 23, 1999·Clinical and Experimental Dermatology·G KirtschigR Happle
Feb 15, 2000·Clinical and Experimental Dermatology·K MolnarH H Pas
Dec 3, 1999·International Journal of Dermatology·C A Egan, J J Zone
Apr 12, 2000·International Journal of Dermatology·C A EganJ J Zone
Mar 16, 2002·International Journal of Dermatology·Keith M BenbenistyLoretta S Davis
Oct 3, 2002·The EMBO Journal·Claus-Werner FranzkeLeena Bruckner-Tuderman
Aug 1, 1995·International Journal of Dermatology·S C Kim
Jan 1, 2008·The Journal of Investigative Dermatology·Michael Hertl, Detlef Zillikens
Sep 11, 2014·The Journal of Investigative Dermatology·Atsunari TsuchisakaTakashi Hashimoto
May 8, 1999·The British Journal of Dermatology·O SwenssonE Christophers
Mar 1, 1994·Clinical and Experimental Dermatology·G Kirtschig, F Wojnarowska
Dec 15, 2012·Lancet·Enno Schmidt, Detlef Zillikens
Feb 12, 2004·The Journal of Investigative Dermatology. Symposium Proceedings·John J ZoneLaurence J Meyer
Dec 6, 2011·Clinics in Dermatology·Giulio Fortuna, M Peter Marinkovich
Feb 11, 1998·The Journal of Investigative Dermatology·L S ChanK B Gordon

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